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Anti-Vimentin Antibody

Name: Anti-Vimentin Antibody
Brand: SIGMA
Price: 469.2 USD
Availability: InStock

mouse monoclonal, V9

Synonym(s):

Anti-Vimentin antibody, Mouse monoclonal

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00 $469.20
200 μL	Available to ship TODAYfromMILWAUKEE	$727.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

V9, monoclonal

Application:

ARR, FACS, ICC, IHC (p), WB

Citations:

135

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Properties

Product Name

Anti-Vimentin antibody, Mouse monoclonal, clone V9, purified from hybridoma cell culture

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

V9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue, microarray: suitable, western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68

isotype

IgG1

UniProt accession no.

P08670

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

Description

General description

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the V9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with vimentin purified from pig eye lens.

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α--helical amino- terminal “head” and carboxy-terminal “tail” domain.

Immunogen

pig eye lens vimentin.

Application

Monoclonal Anti-Vimentin antibody produced in mouse has been used in immunohistochemical analysis and western blot assay.

Monoclonal Anti-Vimentin antibody produced in mouse has been used in:

immunofluorescence
immunohistochemistry
immunocytochemistry
immunoblotting
flow cytometry.

Biochem/physiol Actions

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	SAB4200761	V6630	V2258
Sigma-Aldrich V6389 Anti-Vimentin antibody, Mouse monoclonal	Sigma-Aldrich SAB4200761 Anti-Vimentin antibody, Mouse monoclonal	Sigma-Aldrich V6630 Monoclonal Anti-Vimentin antibody produced in mouse	Sigma-Aldrich V2258 Monoclonal Anti-Vimentin antibody produced in mouse
description clone V9, purified from hybridoma cell culture	description clone LN-6, purified from hybridoma cell culture	description clone V9, ascites fluid	description clone LN-6, ascites fluid
clone V9, monoclonal	clone LN-6, monoclonal	clone V9, monoclonal	clone LN-6, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human	species reactivity rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster	species reactivity pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat	species reactivity pig, mouse, sheep, human, bovine, rabbit, feline, rat
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form ascites fluid
Gene Information human ... VIM(7431) rat ... Vim(81818)	Gene Information bovine ... Vim(280955) dog ... Vim(477991) hamster ... Vim(100689099) human ... VIM(7431) mouse ... Vim(22352) pig ... Vim(100522394) rabbit ... Vim(100008924) rat ... Vim(81818) rhesus monkey ... Vim(705289)	Gene Information human ... VIM(7431) rat ... Vim(81818)	Gene Information human ... VIM(7431) mouse ... Vim(22352) rat ... Vim(81818)
conjugate unconjugated	conjugate -	conjugate unconjugated	conjugate unconjugated

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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