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A3076

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse

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0.2 mL
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€221.00
0.5 mL
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€548.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
GE-1, monoclonal
Application:
DB, ELISA (d)
Citations:
14

€221.00

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biological source

mouse

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Binds human IgE; does not bind human IgG, IgA or IgM.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :
  • immunoblot assays
  • direct ELISA
  • dot blot
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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A2064A2189A9544
Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal clone GE-1, purified from hybridoma cell culture

Sigma-Aldrich

A3076

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal clone GG-5, purified from hybridoma cell culture

Sigma-Aldrich

A2064

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal clone MB-11, purified from hybridoma cell culture

Sigma-Aldrich

A2189

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal

Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A9544

Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

technique(s)

direct ELISA: 1:50,000, dot blot: 1:80,000, immunohistochemistry: suitable, western blot (chemiluminescent): 1:80,000

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

technique(s)

direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

-

biological source

mouse

biological source

mouse

biological source

mouse

biological source

goat

isotype

IgG2b

isotype

IgG1

isotype

IgG2b

isotype

-

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000435600
0000435600
0000214412
0000223732
0000223733

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Related Content

Kyoung Yong Jeong et al.
Clinical and diagnostic laboratory immunology, 11(2), 320-324 (2004-03-12)
Chironomids are widely and abundantly distributed in the vicinity of standing waters. Larvae of Chironomus and some other genera are known to contain hemoglobins, which have been described as a major allergen, and the adults that have no hemoglobins also
Nathalie Acevedo et al.
PloS one, 8(11), e78353-e78353 (2013-11-14)
Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential
Gaurab Sircar et al.
The Journal of biological chemistry, 295(9), 2736-2748 (2019-12-29)
Cyclophilins are structurally conserved pan-allergens showing extensive cross-reactivity. So far, no precise information on cross-reactive IgE-epitopes of cyclophilins is available. Here, an 18-kDa IgE-reactive cyclophilin (Rhi o 2) was purified from Rhizopus oryzae, an indoor mold causing allergic sensitization. Based
View All Related Papers

Global Trade Item Number

SKUGTIN
A3076-.5ML04061833360279
A3076-.2ML04061833360262

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