This product is not tested for bacterial DNA content. It contains two components: JumpStart Taq Antibody (A5600) and Dilution Buffer for JumpStart Taq Antibody (B6808). DNase and RNase levels are not detected in JumpStart Taq Antibody, while the Dilution Buffer is not tested for DNase and RNase levels.
A7721
JumpStart™ Taq Antibody
Adds hot-start capabilities to any Taq DNA Polymerase
Synonym(s):
Hot start Taq antibody
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| Size/SKU | Availability | Price |
|---|---|---|
200 test | Check Cart for Availability | $357.00 |
500 test | Check Cart for Availability | $661.00 |
About This Item
Conjugate:
unconjugated
Clone:
monoclonal
Application:
PCR, whole genome amplification
Citations:
4
$357.00
Check Cart for Availability
biological source
mouse
Quality Segment
conjugate
unconjugated
clone
monoclonal
form
liquid
feature
hotstart, PCR Additives
concentration
1.1 mg/mL
technique(s)
PCR: suitable, whole genome amplification: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
General description
JumpStart™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.
Application
JumpStart™Taq Antibody is suitable for use in:
- cytochrome blocked nucleic acid (LNA) probe assay
- ligation-mediated polymerase chain reaction (LM-PCR)
- in Real-Time quantitative PCR Analysis of viral titer
- PCR amplification of complex genomic or cDNA templates
- to amplify very low-copy-number targets
- for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube
Packaging
Supplied with dilution buffer.
Preparation Note
Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.
Other Notes
JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials
Dilution Buffer provided in 1 mL vials
JumpStart™ Taq Antibody is for R&D use only.Not for drug, household, or other uses.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description Adds hot-start capabilities to any Taq DNA Polymerase | description with MgCl2 | description without MgCl2 | description Complete optimized reagent for hot-start PCR at 2X concentration |
| clone monoclonal | clone - | clone - | clone - |
| conjugate unconjugated | conjugate - | conjugate - | conjugate - |
| biological source mouse | biological source - | biological source - | biological source - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| technique(s) PCR: suitable, whole genome amplification: suitable | technique(s) whole genome amplification: suitable, PCR: suitable | technique(s) whole genome amplification: suitable, PCR: suitable | technique(s) PCR: suitable, whole genome amplification: suitable |
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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What is the bacterial DNA content in this preparation? What are the levels of DNase and RNase?
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