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A9207

Avidin−Agarose from egg white

aqueous glycerol suspension

Synonym(s):

Avidin agarose

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1 mL
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$183.00
5 mL
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$629.00

About This Item

UNSPSC Code:
41106514
NACRES:
NA.56
MDL number:
MFCD00130572
Form:
aqueous glycerol suspension
Biological source:
egg white

$183.00

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biological source

egg white

Quality Segment

200

conjugate

agarose conjugate

form

aqueous glycerol suspension

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino

matrix spacer

15 atoms

storage temp.

−20°C

General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Application

Avidin-Agarose from egg white has been used:
  • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
  • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
  • in avidin bead assay for purification of labelled biotin
  • for capture of glycoproteins from murine cornea samples

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Other Notes

One unit will bind 1.0 μg of d-biotin.

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This Item
A9275A3151A2050
Avidin−Agarose from egg white aqueous glycerol suspension

Sigma-Aldrich

A9207

Avidin−Agarose from egg white

Avidin from egg white BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

Sigma-Aldrich

A9275

Avidin from egg white

Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–FITC from egg white buffered aqueous solution

Sigma-Aldrich

A2050

Avidin–FITC from egg white

description

aqueous glycerol suspension

description

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

description

lyophilized powder

description

buffered aqueous solution

biological source

egg white

biological source

egg white

biological source

avidin from egg white, enzyme from horseradish

biological source

-

form

aqueous glycerol suspension

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

agarose conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

FITC conjugate

matrix

cross-linked 6% beaded agarose

matrix

-

matrix

-

matrix

-

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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