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Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

Name: Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat
Brand: SIGMA
Price: 324 CAD

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Mouse IgG (whole molecule)−AP

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Pack Size	SKU	Availability	Price
0.25 mL		Please contact Customer Service for Availability	$324.00
1 mL		Please contact Customer Service for Availability	$1,030.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162640

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

$324.00

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Properties

biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Application

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:

histological studies in heart and liver grafts
immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
western blot detection in blood samples

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.

Biochem/physiol Actions

IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with rat samples.

Other Notes

Antibody adsorbed with rat serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	A3562	A3688	A5153
Sigma-Aldrich A9316 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3562 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3688 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A5153 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat
biological source goat	biological source goat	biological source goat	biological source goat
technique(s) direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:7,000-1:21,000
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
A9316-1ML	04061837514760
A9316-.5ML	04061835805822
A9316-.25ML	04061837514753

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