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C6423

α-Chymotrysin Protease

Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas

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25 μg

Available to ship TODAYfromMILWAUKEE

$146.00
0.1 mg

Available to ship TODAYfromMILWAUKEE

$437.00

About This Item

CAS Number:
9004-07-3
UNSPSC Code:
12352204
eCl@ss:
42010112
NACRES:
NA.26
EC Number:
232-671-2
MDL number:
MFCD00130481
EC Number:
3.4.21.1 (BRENDA, IUBMB)

$146.00

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Product Name

α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder

grade

Proteomics Grade

Quality Segment

200

form

salt-free, lyophilized powder

mol wt

25 kDa

suitability

suitable for protein sequencing

UniProt accession no.

P00767

storage temp.

2-8°C

Gene Information

cow ... CTRB1(618826)

General description

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein.[1] The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.[2]
Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Application

α-Chymotrypsin from bovine pancreas is used for the following applications:
  • Protein Identification by mass spectrometry (MS)[3]
  • The isolation and characterization of myosin heavy chains[4]
  • Toxin preparation[5]
  • The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE[6]
α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
α-Chymotrypsin from bovine pancreas is stabilized by Ca2+ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu2+ and Hg.

Other Notes

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

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This Item
C7762C4129C3142
α-Chymotrypsin from bovine pancreas suitable for protein sequencing, salt-free, lyophilized powder

Sigma-Aldrich

C6423

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin from bovine pancreas Type I-S, essentially salt-free, lyophilized powder

Sigma-Aldrich

C7762

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin from bovine pancreas Type II, lyophilized powder, ≥40 units/mg protein

Sigma-Aldrich

C4129

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin from bovine pancreas (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

Sigma-Aldrich

C3142

α-Chymotrypsin from bovine pancreas

description

suitable for protein sequencing, salt-free, lyophilized powder

description

Type I-S, essentially salt-free, lyophilized powder

description

Type II, lyophilized powder, ≥40 units/mg protein

description

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

grade

Proteomics Grade

grade

-

grade

-

grade

-

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

suitability

suitable for protein sequencing

suitability

-

suitability

-

suitability

-

form

salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

mol wt

25 kDa

mol wt

25 kDa

mol wt

25 kDa

mol wt

25 kDa

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

P00767

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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