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C6423

α-Chymotrysin Protease

Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas

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25 μg

Estimated to ship TODAYfromMILWAUKEE

$146.00
0.1 mg

Estimated to ship TODAYfromMILWAUKEE

$437.00

About This Item

CAS Number:
9004-07-3
UNSPSC Code:
12352204
eCl@ss:
42010112
NACRES:
NA.26
EC Number:
232-671-2
MDL number:
MFCD00130481
EC Number:
3.4.21.1 (BRENDA, IUBMB)

$146.00

Estimated to ship TODAYDetails

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Product Name

α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder

grade

Proteomics Grade

Quality Segment

200

form

salt-free, lyophilized powder

mol wt

25 kDa

suitability

suitable for protein sequencing

UniProt accession no.

P00767

storage temp.

2-8°C

Gene Information

cow ... CTRB1(618826)

General description

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.
Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Application

α-Chymotrypsin from bovine pancreas is used for the following applications:
  • Protein Identification by mass spectrometry (MS)
  • The isolation and characterization of myosin heavy chains
  • Toxin preparation
  • The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE
α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
α-Chymotrypsin from bovine pancreas is stabilized by Ca2+ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu2+ and Hg.

Other Notes

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

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This Item
CHY5SC9134T8658
α-Chymotrypsin from bovine pancreas suitable for protein sequencing, salt-free, lyophilized powder

Sigma-Aldrich

C6423

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin from bovine pancreas ≥40 units/mg protein, vial of 5 mg

Sigma-Aldrich

CHY5S

α-Chymotrypsin from bovine pancreas

α-Chymotrypsin−Agarose from bovine pancreas lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

Sigma-Aldrich

C9134

α-Chymotrypsin−Agarose from bovine pancreas

Trypsin from bovine pancreas suitable for protein sequencing, lyophilized powder

Sigma-Aldrich

T8658

Trypsin from bovine pancreas

description

suitable for protein sequencing, salt-free, lyophilized powder

description

≥40 units/mg protein, vial of 5 mg

description

lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

description

suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

grade

-

grade

-

grade

Proteomics Grade

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

Gene Information

cow ... CTRB1(618826)

Gene Information

-

form

salt-free, lyophilized powder

form

solid

form

lyophilized powder

form

lyophilized powder

suitability

suitable for protein sequencing

suitability

-

suitability

-

suitability

suitable for protein sequencing

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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