The amplified DNA should be loaded onto an agarose gel after the PCR is completed by adding a separate loading buffer or tracking dye, such as Gel Loading Solution, Catalog Number G2526.
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1.2 mL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 185,00 € |
12 mL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 1720,00 € |
125 mL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 11.560,00 € |
Informazioni su questo articolo
185,00 €
Quality Segment
usage
sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable, whole genome amplification: suitable
color
colorless
input
crude DNA
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
Application
Legal Information
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Questo articolo | |||
|---|---|---|---|
| description Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue | description Ready-to-use 2X PCR Master Mix with Loading Dye | description Complete PCR reagent with standard Taq DNA Polymerase and inert dye | description for PCR |
| technique(s) PCR: suitable | technique(s) - | technique(s) PCR: suitable | technique(s) PCR: suitable |
| feature dNTPs included, Direct PCR, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart: no, Standard PCR | feature dNTPs included, hotstart |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| color colorless | color red | color red | color red |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| input crude DNA | input - | input purified DNA | input purified DNA |
Classe di stoccaggio
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sehr geehrte Damen und Herren, sollen wir nach dem PCR Lauf, Ladepuffer dazugeben?
1 risposta-
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Can I use the TriTrack DNA Loading Dye or the TrakitCyanoOrange Loading Buffer with the ReadyMix??
1 risposta-
The choice of loading dye will not impact the performance the the E3004 - Extract-N-Amp™ PCR ReadyMix. While we have not tested loading dyes outside of the product, G2526, any commercial DNA loading dye is expected to be compatible.
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What is the polymerase used for this kit? I am trying to calculate Tm for my PCR, but I would need to know what type of polymerase this kit uses. I am guessing something close to Q5 Hotstart?
1 risposta-
This kit employs Taq DNA polymerase (Product D6677).
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