This enzyme is lyophilized from a solution containing buffer salts and a stabilizer. The product may be reconsituted at 1 mg/mL in water. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/412/974/g7646dat.pdf
G7646
β-Glucuronidase from Escherichia coli
Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
Synonym(s):
β-
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| Size/SKU | Availability | Price |
|---|---|---|
5000 units | Check Cart for Availability | €58.80 |
25000 units | Check Cart for Availability | €130.00 |
100000 units | Check Cart for Availability | €419.00 |
500000 units | Check Cart for Availability | €1,430.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
€58.80
Check Cart for Availability
type
Type VII-A
Quality Segment
form
lyophilized powder
specific activity
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
mol wt
290 kDa
composition
Protein, ~25% biuret
storage temp.
−20°C
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Contains buffer salts and stabilizer.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
1 of 1
This Item | |||
|---|---|---|---|
| description Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay) | description Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay) | description ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder | description aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret) |
| specific activity 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay) | specific activity 1,000,000-5,000,000 units/g protein (30 min assay) | specific activity ≥10,000,000 units/g protein (30 min assay) | specific activity ≥5,000,000 units/g protein, pH 6.8 (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form aqueous glycerol solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 290 kDa | mol wt 290 kDa | mol wt ~290 kDa | mol wt 69-71 kDa |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| type Type VII-A | type Type IX-A | type - | type - |
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signalword
Danger
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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It is recommended to resuspend the product in which buffer?
1 answer-
Helpful?
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Is BSA or other protein components included in the overall percentage listed on the CoA for the product G7646, which is β-Glucuronidase from Escherichia coli (CAS 9001-45-0)?
1 answer-
No. BSA or other protein sources are added to the product except Potassium Phosphate and Sucrose. Although this is a fermentation product, there may be other protein residues in it in a negligible amount. The product is tested by activity, residual protein contaminants are not tested. The protein percentage is the total protein (tested by Biuret method).
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