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β-Glucuronidase from Escherichia coli

Name: β-Glucuronidase from <I>Escherichia coli</I>
Brand: SIGMA

aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.31 (BRENDA, IUBMB)

MDL number:

MFCD00199013

Specific activity:

≥5,000,000 units/g protein, pH 6.8 (biuret)

Biological source:

Escherichia coli

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Properties

biological source

Escherichia coli

Quality Segment

200

form

aqueous glycerol solution

specific activity

≥5,000,000 units/g protein, pH 6.8 (biuret)

mol wt

69-71 kDa

shipped in

wet ice

storage temp.

−20°C

Description

General description

β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.

Application

β-Glucuronidase from Escherichia coli has been used in the enzymatic cleavage and activation of glucuronide prodrugs in non-small cell lung cancer cells, U87 human glioblastoma cell line, in A549 (human lung adenocarcinoma) and KB (human oral squamous carcinoma).

β-Glucuronidase from E. coli is used for the enzymatic hydrolysis of b-glucuronides in urine and other fluids. It has a high rate of hydrolytic activity and may be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.
The optimal conditions for the enzymatic hydrolysis of α-hydroxytriazolam, one of the major metabolites of triazolam in human urine, were determined using β-glucuronidase Type IX-A.
It is used as a reporter gene in GUS assays to monitor gene expression.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Biochem/physiol Actions

β-Glucuronidase catalyzes the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase is a potential candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. Use of β-Glucuronidase-albumin complex based drug delivery could be an effective therapeutic methodology for treating solid tumors.

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Physical form

Highly purified solution in 50% glycerol

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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This Item	G7646	G8295	G7396
Sigma-Aldrich G8162 β-Glucuronidase from Escherichia coli	Sigma-Aldrich G7646 β-Glucuronidase from Escherichia coli	Sigma-Aldrich G8295 β-Glucuronidase from Escherichia coli	Sigma-Aldrich G7396 β-Glucuronidase from Escherichia coli
description aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)	description Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)	description ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder	description Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
biological source Escherichia coli	biological source -	biological source -	biological source -
specific activity ≥5,000,000 units/g protein, pH 6.8 (biuret)	specific activity 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)	specific activity ≥10,000,000 units/g protein (30 min assay)	specific activity 1,000,000-5,000,000 units/g protein (30 min assay)
form aqueous glycerol solution	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 69-71 kDa	mol wt 290 kDa	mol wt ~290 kDa	mol wt 290 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in -	shipped in wet ice

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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