Hyaluronidase is soluble in water, however most aqueous buffers will be suitable. For testing purposes the bovine enzyme is reconstituted in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0. Solutions are stable up to 1 month refrigerated at 4 deg. C. For long term storage, maintain a concentration of >1 mg/mL, aliquot and freese at - 20 deg. C. Avoid repeated freeze/thaw cycles.
H6254
Hyaluronidase from sheep testes
Type V, lyophilized powder, ≥1,500 units/mg solid
Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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| Size/SKU | Availability | Price |
|---|---|---|
500 mg | Available to ship TODAYfromMILWAUKEE | $566.00 |
1 g | Check Cart for Availability | $982.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
Specific activity:
≥1,500 units/mg solid
Biological source:
sheep testis
biological source
sheep testis
Quality Segment
type
Type V
form
lyophilized powder
specific activity
≥1,500 units/mg solid
mol wt
55 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
General description
Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.
Application
Hyaluronidase has been used:
- as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
- in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
- in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines
Biochem/physiol Actions
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
1 of 1
This Item | |||
|---|---|---|---|
| description Type V, lyophilized powder, ≥1,500 units/mg solid | description Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid | description Type VIII, lyophilized powder, 300-1,000 U/mg | description Type I-S, lyophilized powder, 400-1000 units/mg solid |
| biological source sheep testis | biological source bovine testis | biological source bovine testis | biological source - |
| specific activity ≥1,500 units/mg solid | specific activity 750-3000 units/mg solid | specific activity 300-1,000 U/mg | specific activity 400-1000 units/mg solid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
| form lyophilized powder | form lyophilized powder (essentially salt-free) | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 55 kDa | mol wt ~55 kDa (four subunits of 14 kDa each) | mol wt ~55 kDa (four subunits of 14 kDa each) | mol wt ~55 kDa (four subunits of 14 kDa each) |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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