Hyaluronidase is soluble in water, however most aqueous buffers will be suitable. For testing purposes the bovine enzyme is reconstituted in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0. Solutions are stable up to 1 month refrigerated at 4 deg. C. For long term storage, maintain a concentration of >1 mg/mL, aliquot and freese at - 20 deg. C. Avoid repeated freeze/thaw cycles.
H6254
Hyaluronidase from sheep testes
Type V, lyophilized powder, ≥1,500 units/mg solid
Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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500 MG
$529.00
1 G
$918.00
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
sheep testis
Quality Level
type
Type V
form
lyophilized powder
specific activity
≥1,500 units/mg solid
mol wt
55 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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General description
Application
Hyaluronidase has been used:
- as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules[3]
- in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry[4]
- in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines[5]
Biochem/physiol Actions
Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.[1]
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Unit Definition
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protocols
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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