H6254

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• Enzyme Commission number: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• NACRES: NA.54

Properties

• biological source: sheep testis
• Quality Level: 200
• type: Type V
• form: lyophilized powder
• specific activity: ≥1,500 units/mg solid
• mol wt: 55 kDa
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

General description

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Application

Hyaluronidase has been used:

• as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
• in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
• in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Biochem/physiol Actions

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves