This product is an ascites fluid. The IgG2b concentration may range from 1.0 - 3.0 mg/mL. This information is reported in the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i9388#product-documentation
I9388
Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse
clone HP-6001, ascites fluid
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| Size/SKU | Availability | Price |
|---|---|---|
0.2 mL | Available to ship TODAYfromMILWAUKEE | $236.00 |
0.5 mL | Available to ship TODAYfromMILWAUKEE | $442.00 $375.70 |
About This Item
Conjugate:
unconjugated
Clone:
HP-6001, monoclonal
Application:
ELISA (i)
Citations:
13
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
HP-6001, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:3,000
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Monoclonal Anti-Human IgG1 (Fc specific) antibody is specific for an epitope in the Fc region of human IgG1 and does not detect IgG2, IgG3, and IgG4. The association constant of this antibody with its ligand is estimated to be 1x(107) L/M. This product clone has been tested for specificity using various immunoassays in the IUIS/WHO study.
Monoclonal Anti-Human IgG1 (Fc specific) antibody is specific for an epitope in the Fc region of human IgG1 and does not detect IgG2, IgG3, and IgG4. The association constant of this antibody with its ligand is estimated to be 1x(107) L/M. This product clone has been tested for specificity using various immunoassays in the IUIS/WHO study.
Immunogen
Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads
Application
Monoclonal Anti-Human IgG1 (Fc specific) antibody is suitable for use in ELISA and immunoblot.
Biochem/physiol Actions
IgG1 has a molecular weight of 146 kDa and is an abundantly expressed IgG subclass. IgG1 deficiencies contribute to overall decrease in total IgG levels resulting in hypogammaglobulinemia. IgG1 promotes bacterial phagocytosis and mediates vaccine-induced protection from infection. The ratio of IgG2a to IgG1 is crucial for immune response against Leishmania tropica infection. It has high binding affinity towards the Fcγ receptors (FcγR).
Monoclonal Anti-Human IgG1 (Fc specific) is specific for the IgG1 subclass and nonreactive with IgG2, IgG3, and IgG4 in an ELISA. The antibody recognizes an epitope expressed in the Fc region of IgG1. The estimated association constant of this antibody with its ligand is 1x107 L/M.
Physical form
Monoclonal Anti-Human IgG1 (Fc specific) is provided as ascites fluid with 15 mM sodium azide.
Preparation Note
Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone HP-6001, ascites fluid | description clone GG-7, ascites fluid | description clone HP-6014, ascites fluid | description affinity isolated antibody, buffered aqueous glycerol solution |
| technique(s) indirect ELISA: 1:3,000 | technique(s) indirect ELISA: 1:40,000 | technique(s) indirect ELISA: 1:5,000 | technique(s) western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, direct ELISA: 1:40,000 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate alkaline phosphatase conjugate |
| antibody form ascites fluid | antibody form ascites fluid | antibody form ascites fluid | antibody form affinity isolated antibody |
| biological source mouse | biological source mouse | biological source mouse | biological source goat |
| isotype IgG2b | isotype IgG1 | isotype IgG1 | isotype - |
| clone HP-6001, monoclonal | clone GG-7, monoclonal | clone HP-6014, monoclonal | clone polyclonal |
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Storage Class
12 - Non Combustible Liquids
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Which is the concentration of the antibody? It only states the volume
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