NA0800
GenElute™ HP Select Plasmid Gigaprep Kit
sufficient for 5 preparations
Sustainability features
Synonym(s):
HP gigaprep kit, endo free gigaprep kit, endotoxin free giga kit, endotoxin free plasmid kit, fast gigaprep kit, fast plasmid isolation, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation, Gen Elute, GenElute™ HP Plasmid Kit
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $931.00 |
About This Item
$931.00
usage
sufficient for 5 preparations
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product, Sustainability features
technique(s)
DNA extraction: suitable
test parameters
sample volume: 2000 mL bacterial culture
storage temp.
15-25°C
General description
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water.
The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines.
Application
Features and Benefits
- Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg
- Only 90 minutes from pelleted cells to purified plasmid
- Vacuum format with no ethanol precipitation
- Can purify low-, medium-, and high-copy plasmid DNA
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 5 preparations | description 1 kit sufficient for 5 preparations | description sufficient for 10 preparations | description sufficient for 350 preparations |
| technique(s) DNA extraction: suitable | technique(s) - | technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable |
| usage sufficient for 5 preparations | usage kit sufficient for 5 preparations | usage sufficient for 10 preparations | usage sufficient for 350 preparations |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| greener alternative category | greener alternative category , Aligned | greener alternative category | greener alternative category |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| test parameters sample volume: 2000 mL bacterial culture | test parameters sample volume: 200-1000 mL bacterial culture | test parameters sample volume: 150 mL bacterial culture | test parameters : <30 min kit run time, sample volume: 1-5 mL bacterial culture |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
Target Organs
Central nervous system, Lungs
Storage Class
3 - Flammable liquids
Flash Point (°F)
77.0 °F - closed cup
Flash Point (°C)
25 °C - closed cup
WGK
WGK 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.