The cyanogen bromide-activated agarose beads used to produce this product are 40-165 µm in diameter. Please see the link below to review additional information, including product preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/199/431/p4406dat.pdf
P4406
Papain Agarose from papaya latex
lyophilized powder, 90-150 units/mL packed gel
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| Size/SKU | Availability | Price |
|---|---|---|
25 units | Check Cart for Availability | $336.00 |
50 units | Check Cart for Availability | $647.00 |
100 units | Check Cart for Availability | $1,070.00 |
About This Item
Specific activity:
90-150 units/mL packed gel
Biological source:
papaya (latex)
biological source
papaya (latex)
Quality Segment
form
lyophilized powder
specific activity
90-150 units/mL packed gel
mol wt
21 kDa
extent of labeling
3,000-5,000 units per g agarose (at pH7.0 at 30 °C.)
matrix
beaded agarose
storage temp.
−20°C
Application
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Physical form
Lyophilized powder, stabilized with lactose
Other Notes
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25°C, unless otherwise indicated below.
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This Item | |||
|---|---|---|---|
| description lyophilized powder, 90-150 units/mL packed gel | description lyophilized powder, aseptically filled | description crude powder, 1.5-10 units/mg solid | description lyophilized powder, ≥10 units/mg protein |
| biological source papaya (latex) | biological source papaya (latex) | biological source - | biological source papaya (latex) |
| specific activity 90-150 units/mL packed gel | specific activity ≥8.0 units/mg protein | specific activity 1.5-10 units/mg solid | specific activity ≥10 units/mg protein |
| form lyophilized powder | form lyophilized powder | form crude powder | form lyophilized powder |
| mol wt 21 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Hello, could you please tell me the diameter of the agarose beads used in the product? I couldn't find the info on the store page or in the product description. (product number P4406-25UN) Best regards, Andria
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