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Stem Cell Factor from mouse

Name: Stem Cell Factor from mouse
Brand: SIGMA
Price: 520 USD
Availability: InStock

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):

SCF, c-Kit ligand

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Size/SKU	Availability	Price
10 μg	Check Cart for Availability	$520.00
50 μg	Available to ship TODAYfromMILWAUKEE	$1,230.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00132378

Biological source:

mouse

Recombinant:

expressed in E. coli

Assay:

≥95% (SDS-PAGE and HPLC)

Form:

powder

Mol wt:

18.3 kDa

Impurities:

<1 EU/μg

$520.00

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Properties

Product Name

Stem Cell Factor from mouse, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

mouse

Quality Segment

200

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE and HPLC)

form

powder

potency

≤10 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

18.3 kDa

packaging

pkg of 10 μg, pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

color

white

solubility

water: soluble

UniProt accession no.

P20826

storage temp.

−20°C

Gene Information

mouse ... Kitl(17311), kitl(17311)

Description

General description

Stem cell factor (SCF), also known as the c-kit ligand, is produced by the gastrointestinal smooth muscle cells, enteric neurons, endothelial cell, lung fibroblast and bone marrow adipocytes. The SCF gene is mapped to murine chromosome 10.
Recombinant mouse stem cell factor (SCF) is a 18.3 kDa protein containing 164 amino acid residues.

Application

Stem Cell Factor from mouse has been used in the evaluation of in vitro 5-lipoxygenase inhibition by measuring the formation of LTC4.

SCF, also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Biochem/physiol Actions

Stem cell factor is responsible for cellular development, proliferation, migration and survival, specially observed with cardiac stem cells. This phenomenon is mediated by stem cell factor (SCF) through chemotaxis. SCF is involved in repair mechanism of myocardial infarctions. SCF signalling is responsible for the function and survival rate of interstitial cells of Cajal. Decreased level of SCF leads to gastrointestinal motility disorders. SCF might be associated with systemic vascular remodeling. SCF signalling is also believed to contribute to the pathogenesis of pulmonary hypertension.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

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This Item	S7901	H8416	SRP3234
Sigma-Aldrich S9915 Stem Cell Factor from mouse	Sigma-Aldrich S7901 Stem Cell Factor human	Sigma-Aldrich H8416 Stem Cell Factor human	Sigma-Aldrich SRP3234 SCF from mouse
description SCF, recombinant, expressed in E. coli, powder, suitable for cell culture	description SCF, recombinant, expressed in E. coli, powder, suitable for cell culture	description SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
assay ≥95% (SDS-PAGE and HPLC)	assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)
biological source mouse	biological source human	biological source human	biological source mouse
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
form powder	form powder	form lyophilized powder	form lyophilized

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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