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T3568

TrypZean® bovine

recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid

Synonym(s):

Trypsin bovine

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10 mg
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$343.00
100 mg
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$1,700.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
EC Number:
Specific activity:
≥3,350 units/mg solid
Recombinant:
expressed in corn

$343.00


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recombinant

expressed in corn

Quality Segment

form

lyophilized powder

specific activity

≥3,350 units/mg solid

UniProt accession no.

storage temp.

−20°C

Gene Information

cow ... TRYP8(282603)

General description

TrypZean® eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.

Application

TrypZean® has been used in a study to assess the use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. TrypZean® has also been used in a study to compare a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos.
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.

Biochem/physiol Actions

For dissociation of cultured cells.

Preparation Note

Manufactured by Applied Biotechnology Institute utilizing proprietary transgenic plant protein expression system.

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

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This Item
T6763T3449T1143
description

recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid

description

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

description

recombinant, expressed in corn, sterile-filtered

description

essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)

specific activity

≥3,350 units/mg solid

specific activity

≥8,500 BAEE units/mg protein (biuret)

specific activity

-

specific activity

≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)

Gene Information

cow ... TRYP8(282603)

Gene Information

cow ... TRYP8(282603)

Gene Information

-

Gene Information

bovine ... TRYP8(282603)

form

lyophilized powder

form

powder

form

aqueous solution

form

essentially salt-free, lyophilized powder

recombinant

expressed in corn

recombinant

-

recombinant

expressed in corn

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

Q29463

UniProt accession no.

Q29463

UniProt accession no.

-

UniProt accession no.

Q29463


Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

  1. What should be the working concentration (mg/ml)? thank you!

    1 answer
    1. For the dissociation of monolayer cultures, the suggested working concentration is 0.0025%. The working concentration for use with solid tissue has not been established. The concentration should be optimized by the end user.

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