For the dissociation of monolayer cultures, the suggested working concentration is 0.0025%. The working concentration for use with solid tissue has not been established. The concentration should be optimized by the end user.
T3568
TrypZean® bovine
recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid
Synonym(s):
Trypsin bovine
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| Size/SKU | Availability | Price |
|---|---|---|
10 mg | Check Cart for Availability | $331.00 |
100 mg | Check Cart for Availability | $1,640.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
EC Number:
Specific activity:
≥3,350 units/mg solid
Recombinant:
expressed in corn
$331.00
Check Cart for Availability
recombinant
expressed in corn
Quality Segment
form
lyophilized powder
specific activity
≥3,350 units/mg solid
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... TRYP8(282603)
General description
TrypZean® eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
Application
TrypZean® has been used in a study to assess the use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. TrypZean® has also been used in a study to compare a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos.
Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
Biochem/physiol Actions
For dissociation of cultured cells.
Preparation Note
Manufactured by Applied Biotechnology Institute utilizing proprietary transgenic plant protein expression system.
Other Notes
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.
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This Item | |||
|---|---|---|---|
| description recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid | description Type V-S, ≥8,500 BAEE units/mg protein (biuret) | description essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin) | description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris |
| specific activity ≥3,350 units/mg solid | specific activity ≥8,500 BAEE units/mg protein (biuret) | specific activity ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin) | specific activity ≥10,000 units/mg protein |
| Gene Information cow ... TRYP8(282603) | Gene Information cow ... TRYP8(282603) | Gene Information bovine ... TRYP8(282603) | Gene Information - |
| form lyophilized powder | form powder | form essentially salt-free, lyophilized powder | form lyophilized powder |
| recombinant expressed in corn | recombinant - | recombinant - | recombinant expressed in Pichia pastoris |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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What should be the working concentration (mg/ml)? thank you!
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