Product T3449 is prepared from a 0.0025% concentration.
T3449
TrypZean® Solution, 1×
recombinant, expressed in corn, sterile-filtered
Synonym(s):
Trypsin
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Product Name
TrypZean® Solution, 1×, recombinant, expressed in corn, sterile-filtered
recombinant
expressed in corn
sterility
sterile-filtered
form
aqueous solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
7.70-8.30
shipped in
dry ice
storage temp.
−20°C
Quality Level
1 of 4
This Item | T2601 | C5789 | C5914 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 1 × | concentration 1X | concentration 1 × | concentration 1 × |
| recombinant expressed in corn | recombinant - | recombinant - | recombinant - |
| form aqueous solution | form - | form solution | form liquid |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C |
Application
Features and Benefits
TrypZean® Solution provides the following features and benefits:
- Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
- TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
- Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
- High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
- Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines
General description
TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Samia Rourou et al.
Biotechnology progress, 25(6), 1752-1761 (2009-09-22)
This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that
Maria T Nelson et al.
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Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Katja Peschke et al.
EMBO molecular medicine, 14(4), e14876-e14876 (2022-02-05)
Despite the advance and success of precision oncology in gastrointestinal cancers, the frequency of molecular-informed therapy decisions in pancreatic ductal adenocarcinoma (PDAC) is currently neglectable. We present a longitudinal precision oncology platform based on functional model systems, including patient-derived organoids
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Reinforcement Learning Recruits Somata and Apical Dendrites across Layers of Primary Sensory Cortex.
Clay O Lacefield et al.
Cell reports, 26(8), 2000-2008 (2019-02-21)
The mammalian brain can form associations between behaviorally relevant stimuli in an animal's environment. While such learning is thought to primarily involve high-order association cortex, even primary sensory areas receive long-range connections carrying information that could contribute to high-level representations.
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