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Transforming Growth Factor-β1 human

Name: Transforming Growth Factor-β1 human
Brand: SIGMA
Price: 379 USD
Availability: InStock

TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture

Synonym(s):

TGF-β1, hTGF-β1, Differentiation inhibiting factor, cartilage-inducing factor

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Size/SKU	Availability	Price
2 μg	Estimated to ship onOctober 18, 2027fromMILWAUKEE	$379.00
50 μg	Estimated to ship TODAYfromSAINT LOUIS	$661.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00166089

Biological source:

human

Recombinant:

expressed in CHO cells

Assay:

≥95% (SDS-PAGE)

Form:

powder

Mol wt:

dimer (12.8/25.6 kDa (112/224 aa))

Impurities:

≤0.1 EU/μg (LAL test)

$379.00

Estimated to ship onOctober 18, 2027Details

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Properties

biological source

human

Quality Segment

200

recombinant

expressed in CHO cells

assay

≥95% (SDS-PAGE)

form

powder

potency

≤0.5 ng/mL ED₅₀

mol wt

dimer (12.8/25.6 kDa (112/224 aa))

packaging

pkg of 2 μg, pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤0.1 EU/μg (LAL test)

UniProt accession no.

P01137

storage temp.

−20°C

Description

General description

Tgfb1 (transforming growth factor-β1 human) is a 25kDa peptide encoded by the gene mapped to human chromosome 19q13.2. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3.

Application

Transforming Growth Factor-β1 human has been used in

reporter gene assay.
in vitro transfection.
to study the effect of transforming growth factor-β1 (TGF-β1) on gene array analysis of renal cells.
to study the association of TGF-β1 with shear stress-dependent upregulation of tissue inhibitor of metalloproteinase-1 in microvascular endothelial cells.
to study the interaction between TGF-β1 activation and myofibroblast formation.

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, tissue growth, differentiation, and other functions in a wide range of cell types. TGF-β1 plays a fundamental role in differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. All cells possess a specific TGF-β1 receptor. Both transformed and non-neoplastic tissues release transforming growth factors.The multi-modal nature of TGF-β1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Physical form

Lyophilized from a 0.2 µm filtered solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio

Preparation Note

Reconstitution: Sterile 10 mM HCl at 0.1 mg/mL

Analysis Note

The biological activity of TGF-β1 is measured in culture by inhibition of mouse IL-4-dependent proliferation of mouse HT-2 cells.

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This Item	H8541	H8666	11412272001
Sigma-Aldrich T7039 Transforming Growth Factor-β1 human	Sigma-Aldrich H8541 Transforming Growth Factor-β1 human	Sigma-Aldrich H8666 Transforming Growth Factor-β2 human	Roche 11412272001 Transforming Growth Factor-β1, human (hTGF-β1)
description TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture	description TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture	description TGF-β2, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture	description recombinant (CHO cells)
assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay >95% (SDS-PAGE)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
biological source human	biological source human	biological source human	biological source human
recombinant expressed in CHO cells	recombinant expressed in HEK 293 cells	recombinant expressed in HEK 293 cells	recombinant expressed in CHO cells
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level -
form powder	form lyophilized powder	form lyophilized powder	form solution

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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