H8666

Transforming Growth Factor-β2 human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(s): TGF-β2
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00163957

Properties

• Product Name: Transforming Growth Factor-β2 human, TGF-β2, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.5 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 25 kDa (non-glycosylated)
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P61812
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... TGFB2(7042)

Description

Analysis Note

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Biochem/physiol Actions

TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival.[1] TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells.[2] TGF-β is associated with the process of tumor development, progression and metastasis.[3] TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis.[1] TGF-β2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils.[3] TGF-β is also associated with inflammation and wound healing.[4] The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.[5]

Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

General description

Transforming growth factor-β2 (TGF-β2) belongs to the TGF-β superfamily.[6] All threes isoforms (TGF-β1, TGF-β2 and TGF-β3) are known to share similar structure and function.[5] The TGFB2 gene is mapped to human chromosome 1q41 and is localized to extracellular matrix.[7][8] The active form of TGF-β precursor is produced upon proteolytic cleavage. TGF-β1 and TGF-β2 have 70% amino acid sequence identity.[8]

Preparation Note

HumanKine TGF-β2 is expressed in human HEK 293 cells as a mature, non-glycosylated, disulfide-linked homodimer with a predicted molecular mass of approx. 25 kDa.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable