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Recombinant Antibodies for Research

Three pronged antibody is in the left forefront of the image, with smaller antibodies out of focus in the background.

Recombinant antibodies are considered the new standard in the life sciences due to the ability to engineer optimal molecules for biological detection, without the use of animal models. General advantages of recombinant technology include creating antibodies with high specificity and affinity, reproducibility, and customization for your target antigen. Our ZooMAb® recombinant antibodies platform represents an entirely new generation of recombinant monoclonal antibodies that offer superior quality technology, with the added benefit of greener alternatives, including sustainability. 

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Each antibody is validated to reliably perform across multiple applications including western blotting, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry, and ELISAs and recommended for its most used application. To learn more about ZooMAb® recombinant antibody research applications, please visit our ZooMAb® technical resource page.


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A graph showing antibody types' affinity vs. specificity: Traditional Polyclonal (rabbit), Recombinant Monoclonal (rocket), and Monoclonal (mouse).

Superior Quality of Rabbit Monoclonal Antibodies

Our first group of ZooMAb® antibodies are rabbit-derived, which can produce a large variety of desirable antibodies against “difficult” immunogens in mice, including small molecules (compounds of <600 kDa), peptides, and antibodies specific for post-translational modification sites (PTM). By recognizing a higher diversity of epitopes per antigen at the picomolar level with high specificity, rabbit antibodies tend to provide excellent results in both standard assays and staining applications. For additional information for frequently asked questions, please visit our ZooMAb® antibodies FAQ resource page.

Award Winning Innovation

The ZooMAb® recombinant monoclonal antibody platform was awarded the "2020 Innovative Product of the Year" by CiteAb, a leading life science data provider for citations and market data. We continue to push boundaries to help you make your next big discovery, vial after vial.

ZooMAb® Antibody Multipack

Benefits of ZooMAb® Antibody Technology Compared to Traditional Antibodies

  • Maximum Stability and Convenience

    Every ZooMAb® antibody is provided as a lyophilized product, individually packaged to provide long-term stability, faster shipping, and minimize non-value-added costs that typically incur through cold chain handling of liquids, including the thermal insulated packaging used in transportation. Therefore, every ZooMAb® antibody is exceptionally stable to allow for ambient shipping and storage if needed.

  • Preservative-free

    Every ZooMAb® antibody is provided free of biocides like sodium azide or preservatives of animal origin, such as bovine serum albumin (BSA), and finished in a simple phosphate buffered saline (PBS) with trehalose. With ZooMAb® antibodies, complications arising from potentially hazardous materials or local safety restrictions are minimized and allow its use in both in vivo and in vitro applications.

  • Animal-free

    Every ZooMAb® antibody is produced without the harm or sacrifice of animals. The proprietary recombinant expression system eliminates the need for arduous cell fusion and hybridoma construction, while providing greater specificity, affinity and reproducibility than monoclonal counterparts.

  • Multiple Pack Sizes for Your Specific Need

    Every ZooMAb® antibody is pre-aliquoted at 25 µL lyophilized equivalents to maximize product shelf life. Hence, you only purchase what you need and resuspend what you will use for your research.

  • Consistency for Each Application

    ZooMAb® antibodies are recombinantly produced, purified and pre-aliquoted for you. Every antibody is tested in our own laboratory to work in at least 3 applications and ensured to perform the way you would expect it to in Western blotting, immunofluorescence, and other common applications.

ZooMAb® Antibodies Validation

Proper antibody validation is essential for accurate and reproducible results and is helpful in avoiding unreliable results and reducing research efforts. To ensure their specificity and performance the majority of ZooMAb® antibodies are tested in a minimum of three applications. This includes assessing their binding characteristics and evaluating their performance in multiple assays, which is essential for determining their consistent performance across different experimental conditions.  

Validation Data for Anti-OCT4, Clone 4N8 ZooMAb® Rabbit Monoclonal Antibody. 

Western blot of mouse embryonic stem cell lysate using Anti-OCT4, clone 4N8 ZooMAb® Rabbit Monoclonal antibody. There is one line at ~45kDa with an arrow pointing at the line.

Wester Blot
On mouse embryonic stem cell lysate

Immunohistochemistry of embedded human testis tissue sections. Each image has ovals that represent cells in different colors.

Immunohistochemistry
On paraffin embedded human testis tissue sections

The cells are rectangles that cover the image, with ovals in the middle of the rectangles that represent the nucleus.

Immunofluoresence
Analysis of NTERA2 cells with Alexa Fluor® 488 Dye

Flow cytometry graph - the control graph peaks at 10<sup>1</sup> of yellow fluorescence, while the other graph peaks at 10<sup>3.</sup>

Flow Cytometry
Analysis of NTERA2 cells. Grey-control

ZooMAb® Recombinant Antibodies Offer Lot-to-Lot Consistency

ZooMAb® recombinant antibodies are manufactured by cloning antibody genes into expression vectors, which are subsequently introduced into expression hosts to provide animal-free antibody production. It avoids any sequence mutations and ensures high lot-to-lot consistency, which is also rigorously quality tested in multiple applications. We aim to support researchers in obtaining reliable, reproducible results and even offer an antibody guarantee.

Immunocytochemistry Analysis of two Different lots of Anti-FLT4, Clone 2F4 ZooMAb® Rabbit Monoclonal Antibody. Batch 1 (left) and Batch 2 (right) tested on bEnd.3 cell line at 1:100 dilution. 

There are four immunocytochemistry images. The top left image has two cells outlined with two ovals in the middle that represent the nucleus. There are small dots throughout the cells. In the top right image, there is a rectangle that represents the cell with an oval that represents the nucleus. There are small dots throughout the cell. In the bottom images there are small dots that correspond to the dots in the top images

Please Stay in Touch and Share Your Feedback

Use the convenient QR code printed on each ZooMAb® product label to find up to date information on all the newest additions to our ZooMAb® antibody portfolio and share your experience with these products. Our ZooMAb® antibodies are designed and tested with your needs in mind and are backed by our Antibody Guarantee. We stand by the quality of our product with our Antibody Guarantee, if our antibody does not perform in applications described in our documentation, including product listings on our websites, catalogues or data sheets, we will issue a full credit or replacement antibody product.

Don’t See What You Need?

We offer contract ZooMAb® antibody development services to generate antibodies for your desired targets and applications. Take advantage of our extensive expertise in immunogen design and preparation, antibody development and screening, application-specific testing, and antibody manufacturing at the scale that is relevant to your goals.

For Research Use Only. Not for use in diagnostic procedures.

Unless otherwise stated in the Product(s) specifications, any Antibody product is sold for internal research use only and may not be used for any other purpose, which includes but is not limited to, any commercial, diagnostic, or therapeutic use. Our validation processes pertain only to research uses and do not confirm or assure that our antibodies can be used for any unauthorized uses as set forth herein.





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