Yes, a Microcon DNA fast flow device can be used to isolate DNA from a crude cell lysis preparation. However, lysates typically contain heavy particulate contamination, so clarification spins may be necessary to remove particulates before attempting ultrafiltration.
MRCF0R100
Filtro para centrífuga de fluxo rápido para DNA Microcon®
sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding)
Sinônimo(s):
Centrifuge concentrator
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Sobre este item
Código UNSPSC:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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Estamos aqui para ajudarMateriais
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
Nível de qualidade
esterilidade
non-sterile
Características
holdup volume> 10 μL
embalagem
pkg of 100 ea
fabricante/nome comercial
Microcon®
Parâmetros
0.5 mL sample volume
técnica(s)
DNA purification: suitable
RNA purification: suitable
Comprimento
4.50 cm (1.8 in.)
Diâmetro
12.3 mm
área de filtração
0.32 cm2
volume de trabalho
0.5 mL
Condições de expedição
ambient
1 of 4
Este Item | MRCF0R100ET | MRCF0R030 | MRCPRT010 |
|---|---|---|---|
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) |
| feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable |
Descrição geral
Filtros para centrífuga Microcon® oferecem uma concentração eficiente, dessalinização ou troca de tampão para amostras biológicas aquosas para um intervalo de volumes de 10 a 500 μl. A unidade de filtro para centrífuga de fluxo rápido para DNA Microcon® é otimizada para a concentração e recuperação de DNA com tampão de SDS.
Aplicação
A unidade de filtro para centrífuga de fluxo rápido para DNA Microcon com membrana Ultracel foi usada para extrair DNA orgânico regular para comparar os rendimentos de DNA provenientes de métodos de extração alternativos.[1] Ela também foi usada para filtrar o DNA digerido para análise de microscopia eletrônica de DNA genômico.[2]
Características e benefícios
Os filtros para centrífuga Microcon® apresentam vários benefícios:
- alta recuperação para volumes pequenos com rotação reversa
- membrana Ultracel® com baixo potencial de ligação
- processamento rápido
- fatores de concentração de rendimento de <20× para reprodutibilidade consistente
Outras notas
Substitui: 42413
Informações legais
MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Matteo Berti et al.
Nature communications, 11(1), 3531-3531 (2020-07-17)
Homologous recombination (HR) factors were recently implicated in DNA replication fork remodeling and protection. While maintaining genome stability, HR-mediated fork remodeling promotes cancer chemoresistance, by as-yet elusive mechanisms. Five HR cofactors - the RAD51 paralogs RAD51B, RAD51C, RAD51D, XRCC2 and
Conteúdo relacionado
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. The low nonspecific binding characteristics of the Ultracel® membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.
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Can I use a Microcon DNA fast flow device to isolate DNA from a crude cell lysis preparation such a freeze thaw in TE buffer or a chelex 100 extraction?
1 answer-
Helpful?
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The user guide indicates you can't autoclave these microcon, but can you sterilize them in a UV Crosslinker?
1 answer-
The Microcon devices are non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:
1. Reagent Preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter device.
2.Sanitization Protocol:
A. Fill the Microcon device to its published capacity with filter-sterilized 70% ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.
DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.
Helpful?
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Has the color of the Microcon DNA Fast Flow become brighter blue?
1 answer-
Yes, the color of the device has changed. A new blue colorant and clear resin have replaced the original blue reservoir resin, which has been discontinued. Both old and new resin are HP2 Polycarbonate. The functionality of the product has not changed.
Helpful?
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