E8008

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Sinônimo(s):
EDTA solution
Empirical Formula (Hill Notation):
C10H16N2O8
Número CAS:
Peso molecular:
292.24
Beilstein/REAXYS Number:
1716295
Número MDL:
eCl@ss:
39030907
ID de substância PubChem:
NACRES:
NA.75

Nível de qualidade

200

esterilidade

sterile-filtered

linha de produto

BioReagent

forma

solution

concentração

0.02% in DPBS (0.5 mM)

aplicação(ões)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

7.1-7.7

enviado em

wet ice

temperatura de armazenamento

2-8°C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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Descrição geral

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Aplicação

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

forma física

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

storage_class_code

12 - Non Combustible Liquids

WGK Alemanha

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

Ex vivo Lung bioengineering
Lung Stem Cells in the Epithelium and Vasculature, 151-151 (2015)
The genotoxicity of physiological concentrations of chromium (Cr (III) and Cr (VI)) and cobalt (Co (II)): an in vitro study
Figgitt M, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 688(1-2), 53-61 (2010)
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Wang Y, et al.
Stem Cell Research & Therapy, 8(1), 189-189 (2017)
Cerium fluoride nanoparticles protect cells against oxidative stress.
Shcherbakov AB, et al.
Materials Science and Engineering, C, 50, 151-159 (2015)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)

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