Merck
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E8008

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
EDTA solution
Empirical Formula (Hill Notation):
C10H16N2O8
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
Número MDL:
eCl@ss:
39030907
ID de substância PubChem:
NACRES:
NA.75

esterilidade

sterile-filtered

Nível de qualidade

200

linha de produto

BioReagent

forma

solution

concentração

0.02% in DPBS (0.5 mM)

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

7.1-7.7

enviado em

wet ice

temperatura de armazenamento

2-8°C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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Descrição geral

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Aplicação

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

forma física

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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