Merck
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F3165

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sinônimo(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

Nível de qualidade

200

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

clone

M2, monoclonal

forma

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

purificado por

using Protein A

species reactivity

all

concentração

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

Isotipo

IgG1

sequência de imunogênio

DYKDDDDK

enviado em

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.
F1804 is an affinity purified, FLAG M2 antibody, increasing sensitivity in most applications.
Method of purification - Protein A

Imunogênio

FLAG; peptide sequence DYKDDDDK

Aplicação

Monoclonal ANTI-FLAG® M2 antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunofluorescence
  • ELISA
  • EIA
  • chromatin immunoprecipitation
  • electron microscopy
  • flow cytometry
  • supershift assays

Browse additional application references in our FLAG® Literature portal.

Embalagem

5 mg in poly bottle

Nota de preparo

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Armazenamento e estabilidade

Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.

Informações legais

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Casey C Fowler et al.
Nature communications, 10(1), 3684-3684 (2019-08-17)
Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here
Fangzhi Tan et al.
Nature communications, 10(1), 3733-3733 (2019-08-21)
Hearing loss is the most common sensory disorder. While gene therapy has emerged as a promising treatment of inherited diseases like hearing loss, it is dependent on the identification of gene delivery vectors. Adeno-associated virus (AAV) vector-mediated gene therapy has
Jeong Gu Kang et al.
Scientific reports, 9(1), 11960-11960 (2019-08-21)
Despite the increased interest in epigenetic research, its progress has been hampered by a lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now, many attempts to manipulate DNA methylation have been made using drugs but
Hong Zhu et al.
Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small integral membrane protein of the lysosome/late endosome (SIMPLE) account for the rare autosomal-dominant demyelination in CMT1C patients. Understanding the molecular basis of CMT1C pathogenesis is impeded, in part
Aleksandra Pajak et al.
PLoS genetics, 15(7), e1008240-e1008240 (2019-08-01)
The RNA helicase SUV3 and the polynucleotide phosphorylase PNPase are involved in the degradation of mitochondrial mRNAs but their roles in vivo are not fully understood. Additionally, upstream processes, such as transcript maturation, have been linked to some of these

Artigos

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

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