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Merck

F3165

ANTI-FLAG® M2 monoclonal

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

ANTI-FLAG® M2 antibody, Mouse monoclonal

Sinônimo(s):

Anti-ddddk, Anti-dykddddk

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NACRES:
NA.32
UNSPSC Code:
12352203

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Nome do produto

ANTI-FLAG® M2 monoclonal, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

purified by

using Protein A

species reactivity

all

concentration

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Este Item
F9291A9594A8592
ANTI-FLAG® M2 monoclonal clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

ANTI-FLAG® M2 monoclonal

ANTI-FLAG® M2 monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

ANTI-FLAG® M2 monoclonal

ANTI-FLAG® M2 monoclonal clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Sigma-Aldrich

A9594

ANTI-FLAG® M2 monoclonal

ANTI-FLAG® M2-Peroxidase (HRP) monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A8592

ANTI-FLAG® M2-Peroxidase (HRP) monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

CY3 conjugate

conjugate

peroxidase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous glycerol solution

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

form

buffered aqueous glycerol solution

Application

O anticorpo monoclonal ANTI-FLAG® M2 produzido em camundongo foi usado em:
  • imunoblotting
  • imunoprecipitação
  • imunocitoquímica
  • imunofluorescência
  • ELISA
  • IEE
  • imunoprecipitação de cromatina
  • microscopia eletrônica
  • citometria de fluxo
  • Ensaios de superdesvio de mobilidade eletroforética (supershift)

Explore as aplicações de referência adicionais no nosso portal de literatura FLAG®.

General description

O anticorpo anti-Flag M2 é usado para a detecção de proteínas de fusão Flag. Esse anticorpo monoclonal é produzido em camundongo e reconhece a sequência FLAG no terminal N, terminal Met N e terminal C. O anticorpo também é capaz de reconhecer FLAG em um sítio interno. O M2, diferentemente do anticorpo M1, não é dependente de cálcio.
O F3165 é purificado por afinidade usando resina de proteína A, de modo que contém não apenas o anticorpo anti-FLAG M2, mas também pequenas quantidades de IgG de camundongo nativa, aumentando sua sensibilidade na maioria das aplicações.
Método de purificação - Proteína A

Immunogen

FLAG; sequência de peptídeo DYKDDDDK

Other Notes

Vencedor do Prêmio CiteAb 2024 para Fornecedor de Sucesso em Pesquisa sobre Parkinson′

Preparation Note

Armazene o anticorpo não diluído a –20 °C em alíquotas de trabalho. Não é recomendado congelar e descongelar repetidamente.
Observação: Com o tempo, pequenas quantidades de anticorpos purificados podem se precipitar da solução devido a interações hidrofóbicas intermoleculares. Se um precipitado for observado neste produto, centrifugue brevemente o frasco para criar um pélete do precipitado. Retire o volume desejado de solução de anticorpo do sobrenadante límpido para uso. Isso não deve alterar o desempenho do anticorpo purificado em aplicações de Western blot ou imunoprecipitação.
Dilua a solução de anticorpo a partir de 0,5-10 ug/ml em solução salina tamponada com Tris, pH 8,0, com 3% de leite desnatado

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de armazenamento

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
0000512031
0000512031
0000493941
0000493938
0000493940

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Casey C Fowler et al.
Nature communications, 10(1), 3684-3684 (2019-08-17)
Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here
T P Molitor et al.
Oncogenesis, 2, e48-e48 (2013-06-05)
The vaccinia-related kinases (VRKs) comprise a branch of the casein kinase family. VRK1, a ser/thr kinase with a nuclear localization, is the most well-studied paralog and has been described as a proproliferative protein. In lower eukaryotes, a loss of VRK1
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Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small integral membrane protein of the lysosome/late endosome (SIMPLE) account for the rare autosomal-dominant demyelination in CMT1C patients. Understanding the molecular basis of CMT1C pathogenesis is impeded, in part
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Artigos

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Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

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Data Sheet - F3165
EZview Red Protein A and ANTI-FLAG M2 Affinity Gels

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001

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Questions

1–5 of 5 Questions  

  1. Is there a recommended dilution for capture (sandwich) based ELISA?

    1 answer

    1. Monoclonal ANTI-FLAG® M2 may be used in EIA procedures. Typically, a fusion protein containing a FLAG® peptide sequence is directly adsorbed (or otherwise presented) within the wells of a multiwell polystyrene plate. The Monoclonal ANTI-FLAG® M2 antibody may be diluted up to 1:50,000 for subsequent incubation within the plate wells. Detection may be accomplished using Anti-Mouse IgG-Peroxidase (Cat. No. A9044) or equivalent, diluted 1:10,000, followed by an appropriate substrate for visualization.

      Dilutions for use as the capture antibody in an ELISA have not been evaluated. We would recommend that the end-user optimize the protocol following the recommendations found here:
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/elisa/elisa-procedures#antigen_coating

      Helpful?

  2. What is the recommended dilution for conducting western blotting using this antibody?

    1 answer

    1. The recommended dilution for Western Blot is 10 µg/mL. Please see the link below to review additional information, including protocols:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/274/912/f3165dat-ms.pdf

      Helpful?

  3. What is the difference between F1804 and F3165 product?

    1 answer

    1. Product F1804 and F3165 are both Monoclonal ANTI-FLAG M2 antibodies produced in mouse Clone M2, with the same starting material. The distinction between the products lies in their purification methods. F3165 is immunoglobulin purified, while F1804 is immunogen affinity purified. F1804, being more purified, is expected to exhibit reduced background and less non-specific staining compared to F3165. Both products are suitable for western blotting, immunofluorescence, and immunoprecipitation.

      Helpful?

  4. What is the recommended dilution for conducting immunoprecipitation (IP) using the M2 antibody?

    1 answer

    1. The general starting recommendation for conducting immunoprecipitation (IP) using the F3165, anti-FLAG M2 antibody, is 10 μg/mL.

      Helpful?

  5. What type of light chain is present on the FLAG M2 antibody?

    1 answer

    1. Test has not been conducted to determine whether the light chains of the antibody F3165, Monoclonal ANTI-FLAG® M2 antibody produced in mouse, are kappa or lambda. However, based on customer feedback, it appears that the M2 monoclonal antibody has lambda light chains and not kappa. A probe with anti-kappa did not yield a signal, while anti-lambda produced a good signal.

      Helpful?

1 Review This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

Active Filters

  1. New Jersey
    • Review 1
    • Votes 15
    5 out of 5 stars.

    Great Anti Flag primary

    Works well for our western blot analysis of flag tagged recombinant proteins

    Helpful?

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