Merck
Todas as fotos(3)

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P1146

Sigma-Aldrich

Fosfatase, ácida

lyophilized powder, ≥3.0 units/mg solid

Todas as fotos(3)
Sinônimo(s):
(Ótimo de fosfo-hidrolase monoéster ortofosfórica ácida), Fosfatase ácida
Número CAS:
9001-77-8
Número da licença da enzima:
3.1.3.2 (BRENDA, IUBMB)
Número EC:
232-630-9
Número MDL:
MFCD00131847
eCl@ss:
42010102
NACRES:
NA.54

forma

lyophilized powder

Nível de qualidade

300

specific activity

≥3.0 units/mg solid

peso molecular

69 kDa

enviado em

wet ice

temperatura de armazenamento

−20°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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Este Item
SRE0048P1435P3627
Phosphatase, Acid from potato lyophilized powder, ≥3.0 units/mg solid

Sigma-Aldrich

P1146

Phosphatase, Acid from potato

Sigma-Aldrich

Sigma-Aldrich

SRE0048

Phosphatase, Acid from potato

Sigma-Aldrich

Sigma-Aldrich

P1435

Phosphatase, Acid from sweet potato

Phosphatase, Acid from wheat germ ≥0.4 unit/mg solid

Sigma-Aldrich

P3627

Phosphatase, Acid from wheat germ

specific activity

≥3.0 units/mg solid

specific activity

3.0-10.0 unit/mg solid

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

specific activity

≥0.4 unit/mg solid

mol wt

69 kDa

mol wt

-

mol wt

110 kDa

mol wt

58 kDa by gel filtration

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

300

Quality Level

400

Quality Level

300

Quality Level

300

Descrição geral

Acid phosphatase from potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.

Aplicação

Acid phosphatase from potato has been used in a study to assess the potential allergenicity of novel gene products. It has also been used in a study to remove eight phosphate groups from casein at a pH of 7.0.
The activity of potato acid phosphatase in various surfactant medias was examined. Bis(2- ethylhexyl)sodium sulfosuccinate, Brij 35, and SDS at 3mM inhibited phosphatase activity while the surfactant cetyltrimethylammonium bromide enhanced activity.

Ações bioquímicas/fisiológicas

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Definição da unidade

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Exemplo

T1503
Número do produto
-
25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

insira como1.000309185)

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Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, ≥2,000 DEA units/mg protein

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Alkaline Phosphatase solution, from bovine (calf) intestine, 2 units/μg protein, optimum pH 7.5-9.5

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ß-Glucuronidase/aryl sulfatase

Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

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Phosphatase, Alkaline from Escherichia coli

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E0518

C1 Esterase Inhibitor from human plasma

J Lalitha et al.
Biochemistry and molecular biology international, 41(4), 797-803 (1997-04-01)
The effect of three different classes of surfactants viz., anionic, cationic and neutral on catalytic activity of potato acid phosphatase (AcPase) was studied. Anionic surfactants bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS) inhibited AcPase activity completely at 3
P P Waymack et al.
Archives of biochemistry and biophysics, 288(2), 621-633 (1991-08-01)
An acid phosphatase (orthophosphoric monoester phosphohydrolase, acid optimum; EC 3.1.3.2) isoenzyme from wheat germ was purified 7000-fold to homogeneity. The effect of wheat germ sources and their relationship to the isoenzyme content and purification behavior of acid phosphatases was investigated.
Sishuo Cao et al.
Regulatory toxicology and pharmacology : RTP, 63(2), 181-187 (2012-04-17)
With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three
S I Tu et al.
Plant physiology, 88(1), 61-68 (1988-09-01)
Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions
H N Fernley et al.
The Biochemical journal, 107(2), 279-283 (1968-03-01)
1. A purified preparation of alkaline phosphatase from calf-intestinal mucosa was phosphorylated by (32)P-labelled PP(i) at a serine residue on the enzyme. Under the conditions employed, up to 0.15mum-labelled sites were obtained from 1mum-[(32)P]PP(i). 2. The phosphorylated enzyme was labile
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Protocolos

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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