Merck
All Photos(2)

P5726

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 2

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

Synonym(s):
Protease inhibitor

forma

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

Nível de qualidade

300

enviado em

wet ice

temperatura de armazenamento

2-8°C

Aplicação

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.

Ações bioquímicas/fisiológicas

This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.

Características e benefícios

This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Atenção

As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.

Nota de preparo

This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.

Pictogramas

Exclamation markHealth hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Código de classe de armazenamento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Certificate of Analysis

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Certificate of Origin

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