Yes, a Microcon DNA fast flow device can be used to isolate DNA from a crude cell lysis preparation. However, lysates typically contain heavy particulate contamination, so clarification spins may be necessary to remove particulates before attempting ultrafiltration.
MRCF0R100
Microcon® DNA Fast Flow Zentrifugenfilter
sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding)
Synonym(e):
Centrifuge concentrator
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100 EA
CHF 864.00
Über diesen Artikel
UNSPSC-Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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Unterstützung erhaltenMaterialien
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
Qualitätsniveau
Sterilität
non-sterile
Leistungsmerkmale
holdup volume> 10 μL
Verpackung
pkg of 100 ea
Hersteller/Markenname
Microcon®
Parameter
0.5 mL sample volume
Methode(n)
DNA purification: suitable
RNA purification: suitable
Länge
4.50 cm (1.8 in.)
Durchmesser
12.3 mm
Filtrationsfläche
0.32 cm2
Arbeitsvolumen
0.5 mL
Versandbedingung
ambient
Verwandte Kategorien
1 of 4
Dieser Artikel | MRCF0R100ET | MRCF0R030 | MRCPRT010 |
|---|---|---|---|
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) |
| feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable |
Allgemeine Beschreibung
Microcon® Zentrifugenfilter bieten eine effiziente Konzentrierung, Entsalzung oder einen Pufferaustausch von wässrigen biologischen Proben mit einem Volumen im Bereich von 10–500 μl. Der Microcon® DNA Fast Flow Filter ist für die Konzentrierung und Rückgewinnung von genomischer DNA mit SDS-Puffer optimiert.
Anwendung
Microcon DNA Fast Flow Zentrifugenfiltereinheit mit Ultracel-Membran wird zum Extrahieren von normaler organischer DNA eingesetzt, um DNA-Ausbeuten von alternativen Extraktionsverfahren zu vergleichen.[1] Sie wird auch zum Filtrieren der aufgeschlossenen DNA für die Elektronenmikroskopanalyse von genomischer DNA eingesetzt.[2]
Leistungsmerkmale und Vorteile
Microcon® Zentrifugenfilter haben mehrere Vorteile:
- hohe Rückgewinnung für kleine Volumen mit Umkehr-Spin
- Ultracel® Membran mit geringer Bindung
- schnelle Verarbeitung
- ergeben Konzentrationsfaktoren von <20 X für eine konsistente Reproduzierbarkeit
Sonstige Hinweise
Ersetzt: 42413
Rechtliche Hinweise
MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. The low nonspecific binding characteristics of the Ultracel® membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.
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Can I use a Microcon DNA fast flow device to isolate DNA from a crude cell lysis preparation such a freeze thaw in TE buffer or a chelex 100 extraction?
1 answer-
Helpful?
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The user guide indicates you can't autoclave these microcon, but can you sterilize them in a UV Crosslinker?
1 answer-
The Microcon devices are non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:
1. Reagent Preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter device.
2.Sanitization Protocol:
A. Fill the Microcon device to its published capacity with filter-sterilized 70% ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.
DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.
Helpful?
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Has the color of the Microcon DNA Fast Flow become brighter blue?
1 answer-
Yes, the color of the device has changed. A new blue colorant and clear resin have replaced the original blue reservoir resin, which has been discontinued. Both old and new resin are HP2 Polycarbonate. The functionality of the product has not changed.
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