The actual yield of purified FLAG-tagged protein from a 10 cm dish using the CELLMM2 kit will depend on the expression level of the protein in the cells. The kit’s resin can bind up to 600–1000 µg per 1 mL, but typical yields from mammalian cells are often in the 100–500 µg range per dish. For precise yield, measuring the protein concentration in the lysate before and after purification is recommended.
Fortfahren mit
CELLMM2
FLAG® M Purification Kit
For Mammalian expression systems.
Synonym(e):
Anti-ddddk, Anti-dykddddk
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | CHF 962.00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
12352200
CHF 962.00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenQuality Segment
technique(s)
FLAG purification: suitable, protein extraction: suitable
shipped in
wet ice
storage temp.
−20°C
Application
The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Features and Benefits
- Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
- ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
- Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).
Packaging
Sufficient for 3-5 uses of 1-ml affinity purification column.
Other Notes
Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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| description For Mammalian expression systems. | description 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) | description clone M1, purified immunoglobulin, buffered aqueous solution | description - |
| technique(s) protein extraction: suitable, FLAG purification: suitable | technique(s) FLAG purification: suitable | technique(s) western blot: 10 μg/mL, FLAG purification: suitable | technique(s) immunoprecipitation (IP): suitable, FLAG purification: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
Lagerklasse
10 - Combustible liquids
WGK
WGK 3
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What is the approximate yield of protein (in ug) if you start with a one 10 cm dish?
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