Merck
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FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

Verwendung

1 kit sufficient for 50 reactions

Qualitätsniveau

300

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

versandt in

wet ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Anwendung

Browse additional application references in our FLAG® Literature portal.

Leistungsmerkmale und Vorteile

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

Sonstige Hinweise

All prodedures to be performed at 2-8°C.

Rechtliche Hinweise

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • A2220ANTI-FLAG M2 affinity gel 1 mL

  • SAE01943X FLAG peptide 1 mg

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Aquatic Chronic 3 - Eye Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

SDS

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