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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonym(s):
Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO, Methyl Sulfoxide
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
Numéro EC:
Numéro MDL:
ID de substance PubChem:
NACRES:
NA.52

Niveau de qualité

200

Qualité

for molecular biology

Densité de vapeur

2.7 (vs air)

Pression de vapeur

0.42 mmHg ( 20 °C)

Gamme de produits

BioReagent

Essai/Dosage

≥99.9%

Forme

liquid

Température d'inflammation spontanée

573 °F

Durée de conservation

Recommended retest period - 2 years

Expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

Impuretés

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

Couleur

colorless

Indice de réfraction

n20/D 1.479 (lit.)

Point de bulle

189 °C (lit.)

Pf

16-19 °C (lit.)

Solubilité

H2O: miscible (completely)

Densité

1.10 g/mL (lit.)

Absorption

passes test

Adéquation

suitable for molecular biology

Activité étrangère

DNase and RNase, none detected

Temp. de stockage

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Description générale

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Conditionnement

1 L in glass bottle
50, 100, 250, 500 mL in glass bottle

Caractéristiques et avantages

This product is free from DNases, RNases, phosphatases and proteases.

Attention

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Code de la classe de stockage

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Point d'éclair C

87 °C - closed cup

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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