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Merck

E9884

エチレンジアミン四酢酸

ACS reagent, 99.4-100.6%, powder

別名:

(エチレンジニトリロ)四酢酸, EDTA, エダタミル, エチレンジニトリロ四酢酸

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サイズを選択してください

100 G

¥5,010

500 G

¥17,100

1 KG

¥18,100

¥5,010


本日発送予定詳細


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この商品について

化学式:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS番号:
分子量:
292.24
NACRES:
NA.77
PubChem Substance ID:
eCl@ss:
39030907
UNSPSC Code:
12352106
EC Number:
200-449-4
MDL number:
Beilstein/REAXYS Number:
1716295

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製品名

エチレンジアミン四酢酸, ACS reagent, 99.4-100.6%, powder

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

grade

ACS reagent

agency

suitable for SM 4500 - NH3

assay

99.4-100.6%

form

powder

reaction suitability

reagent type: chelator

impurities

≤0.005% insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

ign. residue

≤0.2%

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

water: soluble 0.4 g/L at 20 °C

cation traces

Ca: ≤0.001%
Fe: ≤0.005%
Mg: ≤5 ppm
heavy metals: ≤0.001% (byICP-OES)

Quality Level

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当該品目
ED4005-OP431788
grade

ACS reagent

grade

purified grade

grade

-

grade

-

assay

99.4-100.6%

assay

≥98.5%

assay

≥99.5%

assay

99.995% trace metals basis

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

-

form

powder

form

powder

form

crystalline powder (Fine)

form

powder

impurities

≤0.005% insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

impurities

-

impurities

-

impurities

-

Application

エチレンジアミン四酢酸は以下の用途に使用されています:
  • 細胞分画{155)時の洗浄段階で使用される標識バッファーの一成分として 
  • 創傷郭清やその他の皮膚組織に対するパパイン酵素の影響の検討[1] 
  • フローサイトメトリー分析で使用するための前処理後のラット動脈平滑筋細胞の収集[2]

Biochem/physiol Actions

エチレンジアミン四酢酸(EDTA)は、経消化管的投与が容易ではありません。EDTAナトリウムを静脈内投与すると、低カルシウム血症性テタニーが生じます。[3]血漿又は分子診断法において概して有用で、カルシウムをキレート化することによって作用します。EDTAは、血球計算や細胞の形態学的解析において抗凝固剤として働きます。[4]

General description

エチレンジアミン四酢酸(EDTA)は親水性金属キレート剤であり、金属イオンを不活性な環状金属錯体に変換します。そのため、金属汚染の解消に工業的に応用されています。[5]

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

保管分類

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


最新バージョンのいずれかを選択してください:

試験成績書(COA)

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特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Streaming of proteolytic enzyme solutions for wound debridement: a feasibility study
Yaakobi T, et al.
Wounds : a compendium of clinical research and practice, 16, 201-205 (2004)
Henry's Clinical Diagnosis and Management by Laboratory Methods E-book, 28-28 (2011)
CRC Desk Reference of Clinical Pharmacology, 215-215 (1997)
Adsorption of metal-ethylenediaminetetraacetic acid chelates onto lake sediment
Sillanpaa M and Ramo J
Chemosphere, 45, 881-885 (2001)
Renée S Suen et al.
The American journal of pathology, 179(3), 1549-1559 (2011-07-02)
Because both endothelin-1 (ET-1) and angiotensin II (AngII) are independent mediators of arterial remodeling, we sought to determine the role of ET receptor inhibition in AngII-accelerated atherosclerosis and aortic aneurysm formation. We administered saline or AngII and/or bosentan, an endothelin

資料

オルガノイド培養用製品により、組織または幹細胞に由来する3Dの脳、腸、肺および腫瘍のオルガノイドモデルを作成できます。

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

プロトコル

アニーリングとは、相補的配列を有する2つの一本鎖オリゴヌクレオチドを加熱・冷却して二本鎖を形成させる工程です。

Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.

関連コンテンツ

This page is intended to make it easier to find the consumables you need based on the analytical method you’re using. Methods included on this page come from the EPA, Standard Methods and ASTM.

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.

質問

1–4/4 質問  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      役に立ちましたか?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  3. is the E9884 product a powder product?

    1 回答
    1. As mentioned in the product title, as well as the 'PROPERTIES' section of the product page, this material is in powder form.

      役に立ちましたか?

  4. Hello!! We are aiming to create a 15% solution of EDTA and wondering what the ratio of EDTA to water should be. We had internal guidance that recommended something close to a 1:1 relationship between EDTA and water. What guidance can you give?

    1 回答
    1. A 15% solution would consist of 15 grams of a given material in a total volume of 100mL. For example, to prepare a 15% solution, a partial volume of solvent (in this case, water) is added to a vessel. The compound of interest (in this case EDTA) is added while stirring and then additional solvent is added to bring the final volume to 100ml. There would be slight adjustments needed depending on the specific solvent and the measure being used - weight per volume or weight per weight. Unfortunately, EDTA is not soluble in water at 15%. The addition of a base, such as sodium hydroxide, may increase the solubility, however this has not been verified. Higher solubility rates may also be achievable using a sodium salt form, such as E4884.

      To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section and respond with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

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