This product contains 3.7 g/L of NaHCO3.
D5796
DMEM - high glucose
With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
別名:
DME, DMEM
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|---|---|---|
| 500 mL | 出荷予測2026年4月13日from市川市大町368 | ￥2,670 | |
| 1 L | 出荷予測2026年4月13日from市川市大町368 | ￥4,630 |
この商品について
UNSPSC Code:
12352207
NACRES:
NA.75
製品名
ダルベッコ改変イーグル培地(高グルコース), With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
General description
このDMEM-高グルコース培地は、L-アラニル-L-グルタミン、HEPES、またはピルビン酸ナトリウムなどの添加物（共通修飾）を含まない、1倍の完全培地です。 ピリドキシンがピリドキサールに置換されているオリジナルのDMEM-高組成とは異なります。 ピリドキサールは不安定な培地成分です。
Application
ダルベッコ改変イーグル培地(DMEM)は、イーグル基礎培地(BME)を改変したもので、4倍濃度のアミノ酸とビタミンが含まれています。オリジナルの処方には、グルコース1000 mg/Lが含まれ、胚性マウス細胞の培養に使用されていました。それ以降、マウス、ニワトリの細胞のほか、様々な正常細胞やトランスフォーム細胞の初代培養をサポートするために幾通りかの方法で改変が行われてきました。これらの培地は、L-グルタミンとピルビン酸ナトリウムの組み合わせがそれぞれに異なります。また、グルコース濃度が4500 mg/Lまで引き上げられたことから、「DMEM/High」と命名されました。
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当該品目 | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| components NaHCO3: yes | components L-glutamine: no | components HEPES: no | components NaHCO3: yes |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
プロトコル
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
安定で長期的な遺伝子サイレンシングおよび表現型の変化を達成するための、MISSION shRNAレンチウイルス粒子の形質導入（トランスダクション）の詳細な手順
資料
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
オルガノイド培養用製品により、組織または幹細胞に由来する3Dの脳、腸、肺および腫瘍のオルガノイドモデルを作成できます。
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グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| D5796-500ML | 04061838259332 |
| D5796-24X500ML | 04061838259325 |
| D5796-1L | 04061833586846 |
| D5796-6X500ML | 04061833586860 |
| D5796-6X1L | 04061833586853 |
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What is the NaHCO3 concentration? Conventional cell culture requires 5% of CO2. But it will depend on the NaHCO3 concentration. Does this product use 1.2 or 3.7 g/L?
1 回答-
役に立ちましたか？
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What are the product numbers for DMEM and EMEM used to grow the 293 and 293T cell lines?
1 回答-
To grow the 293 and 293T cells, the following product recommendations are provided:
For the 293 cell line (product number 85120602), ECACC recommends using EMEM media (EBSS) with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), and 10% FCS. ECACC uses the product number M4655 for this purpose.
For the 293T cell line (product number 12022001), ECACC recommends using DMEM media with 2mM Glutamine and 10% Fetal Bovine Serum (FBS). ECACC uses the product number D5796 media for sub-culturing this cell line.
役に立ちましたか？
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What is the conc. of L glutamine
1 回答-
The concentration of L-Glutamine in this product is 0.584 g/L. Please see the link below to review the DMEM Formulation Tables: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/109/d5796for.pdf
役に立ちましたか？
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pH는 몇으로 조정되어 있나요?
1 回答-
The pH specification of this product is 7.0-7.6. The exact value will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D5796/RNBL9771.
役に立ちましたか？
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