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D9170

Sigma-Aldrich

ジメチルスルホキシド

PCR Reagent

別名:
メチルスルホキシド, DMSO
化学式:
(CH3)2SO
CAS番号:
分子量:
78.13
Beilstein:
506008
EC 番号:
MDL番号:
PubChem Substance ID:
NACRES:
NA.25

グレード

PCR Reagent

品質水準

200

蒸気密度

2.7 (vs air)

蒸気圧

0.42 mmHg ( 20 °C)

アッセイ

≥99.5%

形態

liquid

自己発火温度

573 °F

シェルフライフ

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

包装

vial of 1 mL

technique(s)

PCR: suitable

不純物

≤0.1% water
water

colorless

屈折率

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

溶解性

H2O: miscible (completely)

密度

1.10 g/mL (lit.)

適合性

suitable for PCR

application(s)

agriculture

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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関連するカテゴリー

詳細

ジメチルスルホキシドは、フリーラジカル捕捉剤および凍結保存剤であり、二重鎖切断(DSB)において保護作用を発揮します。ジメチルスルホキシド(1~10%)は、鎖再生を加速することが分かっており、脱プリン反応に反して核酸の熱安定性をもたらすと考えられています。PCR共溶媒として、DMSOは特にlong PCRにおける収率の向上に役立ちます。

アプリケーション

ジメチルスルホキシドは、ポリメラーゼ連鎖反応(PCR)混合液の構成要素としておよびPCR促進剤として使用されています。

注意

容易に過冷却状態となり、室温で徐々に再融解します。凝固している場合は室温に放置すると、劣化させることなく液体に戻すことができます。

その他情報

ジメチルスルホキシドの親和性に関する情報につきましは、以下のリンク先をご覧ください:
ジメチルスルホキシドの混和性/不混和性一覧表

保管分類コード

10 - Combustible liquids

WGK

WGK 1

引火点(°F)

188.6 °F - closed cup

引火点(℃)

87 °C - closed cup

個人用保護具 (PPE)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

試験成績書(COA)

原産地証明書(COO)

  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  4. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)

プロトコル

Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

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