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Merck

D9170

ジメチルスルホキシド

PCR Reagent

別名:

DMSO, メチルスルホキシド

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サイズを選択してください

1 AMP

¥14,200

5 AMP

¥50,500

¥14,200


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この商品について

化学式:
(CH3)2SO
CAS番号:
分子量:
78.13
UNSPSC Code:
12191502
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.5%
Technique(s):
PCR: suitable
Application(s):
agriculture
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

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製品名

ジメチルスルホキシド, PCR Reagent

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

SMILES string

CS(C)=O

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

packaging

vial of 1 mL

technique(s)

PCR: suitable

impurities

≤0.1% water
water

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

suitability

suitable for PCR

application(s)

agriculture

Quality Level

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当該品目
34869D8418D5879
application(s)

agriculture

application(s)

food and beverages

application(s)

-

application(s)

-

grade

PCR Reagent

grade

-

grade

for molecular biology

grade

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

assay

≥99.5%

assay

≥99.7%

assay

≥99.9%

assay

≥99.5%

technique(s)

PCR: suitable

technique(s)

HPLC: suitable

technique(s)

DNA sequencing: suitable, PCR: suitable, transfection: suitable

technique(s)

immunoblotting: suitable

solubility

H2O: miscible (completely)

solubility

-

solubility

H2O: miscible (completely)

solubility

H2O: soluble(lit.), acetone: soluble(lit.), benzene: soluble(lit.), chloroform: soluble(lit.), ethanol: soluble(lit.)

Application

ジメチルスルホキシドは、ポリメラーゼ連鎖反応(PCR)混合液の構成要素として[1]およびPCR促進剤として使用されています。[2][3]

Disclaimer

容易に過冷却状態となり、室温で徐々に再融解します。凝固している場合は室温に放置すると、劣化させることなく液体に戻すことができます。

General description

ジメチルスルホキシドは、フリーラジカル捕捉剤および凍結保存剤であり、二重鎖切断(DSB)において保護作用を発揮します。[4]ジメチルスルホキシド(1~10%)は、鎖再生を加速することが分かっており、脱プリン反応に反して核酸の熱安定性をもたらすと考えられています。[2]PCR共溶媒として、DMSOは特にlong PCRにおける収率の向上に役立ちます。[5]

Other Notes

ジメチルスルホキシドの親和性に関する情報につきましは、以下のリンク先をご覧ください:
ジメチルスルホキシドの混和性/不混和性一覧表

保管分類

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)
Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)

質問

1–9/9 質問  
  1. How much DMSO is in each of the vials (D9170)?

    1 回答
    1. As stated in the 'PROPERTIES' section, each vial contains 1 mL. One ampule contains 1 mL of Dimethyl Sulfoxide, and 5 amps include 5 ampules containing 1 mL of Dimethyl Sulfoxide. each.

      役に立ちましたか?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      役に立ちましたか?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  4. Where does DMSO come from?

    1 回答
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      役に立ちましたか?

  5. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 回答
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      役に立ちましたか?

  6. Does DMSO have any synthetic utility?

    1 回答
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      役に立ちましたか?

  7. What is the Department of Transportation shipping information for this product?

    1 回答
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか?

  8. What is the molarity of DMSO?

    1 回答
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      役に立ちましたか?

  9. What is the DMSO dissolved in?

    1 回答
    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      役に立ちましたか?

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