- F7250
F7250
フルオロセインイソチオシアナート, アイソマー I
suitable for protein labeling, ≥90% (HPLC), powder
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品質水準
アッセイ
≥90% (HPLC)
形状
powder
mp
>360 °C (lit.)
溶解性
acetone: 1 mg/mL
蛍光検出
λex 492 nm; λem 518 nm (green)
適合性
suitable for protein labeling
application(s)
diagnostic assay manufacturing
hematology
histology
保管温度
2-8°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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This Item | F6377 | F2456 | F4274 |
|---|---|---|---|
| assay ≥90% (HPLC) | assay - | assay - | assay ≥97.5% (HPLC) |
| form powder | form powder | form powder | form powder |
| mp >360 °C (lit.) | mp >300 °C | mp 320 °C (lit.) | mp >360 °C (lit.) |
| solubility acetone: 1 mg/mL | solubility - | solubility - | solubility acetone: soluble 10 mg/mL |
| fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green) |
| suitability suitable for protein labeling | suitability - | suitability - | suitability - |
詳細
また、アミン基を介してタンパク質に蛍光標識を付けるために広く使用されています。イソチオシアネート基は、タンパク質のアミノ末端および第一級アミンと反応します。抗体やレクチンなどタンパク質の標識に使用されています。
フルオレセインイソチオシアネート異性体Iは、接触感作物質として提案されています。
アプリケーション
生物学的用途には、タンパク質の蛍光標識試薬、タンパク質追跡用の蛍光試薬、および病原体を迅速に同定するための蛍光抗体法の試薬としての使用などがあります。これは、それらのキャピラリー電気泳動（CE）測定時に、ヒト尿中のアンフェタミン、メタンフェタミン、3,4-メチレンジオキシメタンフェタミンおよびP-フェニルエチルアミンの誘導体化試薬として使用される場合があります。これは、蛍光抗体の調製に使用される場合があります。また、in vitro感作試験に使用されました。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1 - Skin Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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