If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
54457
HEPES
≥99.5% (titration), powder, for molecular biology, BioUltra
Sinónimos:
Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)
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MXP 2,024.00
MXP 7,925.00
MXP 26,469.00
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Nombre del producto
HEPES, BioUltra, Molecular Biology, ≥99.5% (T)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
grade
Molecular Biology
product line
BioUltra
assay
≥99.5% (T)
form
powder
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
ign. residue
≤0.1% (as SO4)
loss
≤0.5% loss on drying, 110 °C
pH
5.0-6.5 (25 °C, 1 M in H2O)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
H2O: 1 M at 20 °C, clear, colorless
anion traces
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
λ
1 M in H2O
UV absorption
λ: 260 nm Amax: 0.045
λ: 280 nm Amax: 0.035
Quality Level
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Categorías relacionadas
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Este artículo | PHR1428 | RDD002 | H3375 |
|---|---|---|---|
| assay ≥99.5% (T) | assay - | assay ≥99.5% | assay ≥99.5% (titration) |
| grade for molecular biology | grade certified reference material, pharmaceutical secondary standard | grade anhydrous | grade - |
| Quality Level 200 | Quality Level 300 | Quality Level 100 | Quality Level 400 |
| product line BioUltra | product line - | product line Redi-Dri™ | product line - |
| form powder | form - | form crystalline powder | form crystalline powder |
| solubility H2O: 1 M at 20 °C, clear, colorless | solubility - | solubility water: 500 mg/mL, clear, colorless | solubility water: 500 mg/mL, clear, colorless |
Application
- DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells[1]
- RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)[2]
- Recapture solution to re-capture RNA exosomes from the glycerol gradient[3]
- ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation[4]
- 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors[5]
General description
Other Notes
Comparable product
Clase de almacenamiento
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocolos
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Contenido relacionado
HEPES Specification Comparison
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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