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54457

Sigma-Aldrich

HEPES

BioUltra, for molecular biology, ≥99.5% (T)

Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número de CAS:
Peso molecular:
238.30
Beilstein:
883043
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

200

grado

for molecular biology

línea de producto

BioUltra

ensayo

≥99.5% (T)

formulario

powder

impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

residuo de ign.

≤0.1% (as SO4)

pérdida

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H2O)

intervalo de pH útil

6.8 - 8.2

pKa (25 °C)

7.5

solubilidad

H2O: 1 M at 20 °C, clear, colorless

trazas de anión

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

trazas de catión

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

Absorción UV

λ: 260 nm Amax: 0.045
λ: 280 nm Amax: 0.035

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used as a component of:
  • DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
  • RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
  • Recapture solution to re-capture RNA exosomes from the glycerol gradient
  • ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
  • 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Otras notas

Easily compare specifications for HEPES products with the HEPES specification table.
3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificado de Análisis

Certificado de origen

Sample Preparation of Telomerase Subunits for Crystallization.
Huang J, et al.
Bio-protocol, 5(16) (2015)
Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
Zhang F, et al.
Nature Protocols, 5(3), 439-439 (2010)
The correction of the metabolic parameters of msg-induced obesity in rats by 2-[4-(benzyloxy) phenoxy] acetic acid.
Konopelniuk V, et al.
Journal of Nutrition and Intermediary Metabolism, 13, 1-9 (2018)
Isolation and analysis of nuclear RNA.
J R Nevins
Methods in enzymology, 152, 234-241 (1987-01-01)
RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells.
Domanski M and LaCava J
Bio-protocol, 7(8) (2017)

Protocolos

TRI Reagent® Protocol

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

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