FLAGIPT1

FLAG^® Immunoprecipitation Kit

• Synonym(s): Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• usage: kit sufficient for 50 reactions
• Quality Segment: 300
• analyte chemical class(es): proteins (FLAG® Affinity Gels)
• technique(s): immunoprecipitation (IP): suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Learn more product details in our FLAG^® application portal.

Features and Benefits

• Utilizes highly specific ANTI-FLAG M2 affinity gel
• No preliminary steps or calibrations
• 3xFLAG^™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• A2220

  ANTI-FLAG M2 affinity gel 1 mL

  SDS
• SAE0194

  3X FLAG peptide 1 mg

  SDS

Safety Information

• pictograms: GHS07
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• signalword: Danger
• hcodes: H319,H412,EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2