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Selective Vaporization of Superheated Nanodroplets for Rapid, Sensitive, Acoustic Biosensing.

Selective Vaporization of Superheated Nanodroplets for Rapid, Sensitive, Acoustic Biosensing.

Advanced healthcare materials (2015-06-19)

Rajarshi Chattaraj, Praveena Mohan, Jeremy D Besmer, Andrew P Goodwin

ABSTRACT

Superheated perfluorocarbon nano-droplets exhibit promise as sensitive acoustic biosensors. Aggregation of biotin-decorated lipid-shelled droplets by streptavidin greatly increases the yield of bubbles formed by ultrasound-induced vaporization. Streptavidin is sensed down to 1 × 10(-13) m, with differentiable signal appearing in as little as two minutes, using a scalable assay without washing, processing, or development steps.

MATERIALS

Product Number

Brand

Product Description

SAFC

Tromethamine

Sigma-Aldrich

Sigma 7-9^®, ≥99% (titration), powder or crystals

Sigma-Aldrich

Nitrilotriacetic acid disodium salt, Sigma Grade, ≥99%

Sigma-Aldrich

Tris(hydroxymethyl)aminomethane, ≥99.8%

Sigma-Aldrich

Sodium chloride solution, 0.85%

Sigma-Aldrich

Sodium chloride, 99.999% trace metals basis

Sigma-Aldrich

Sodium chloride, random crystals, 99.9% trace metals basis

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

Sigma-Aldrich

Tris(tert-butoxy)silanol, 99.999%

Sigma-Aldrich

Sodium chloride solution, 0.1 M

Sigma-Aldrich

Trizma^® base, 99.7% (T), puriss. p.a.

Sigma-Aldrich

Trizma^® base, BioUltra, Molecular Biology, ≥99.8% (T)

Sigma-Aldrich

Trizma^® base, ≥99.0% (T)

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

Sigma-Aldrich

Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

Sigma-Aldrich

Trizma^® base, ≥99.9% (titration), crystalline

Sigma-Aldrich

Trizma^® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Sigma-Aldrich

Tromethamine, meets USP testing specifications

Sigma-Aldrich

Sodium chloride, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Sigma-Aldrich

Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

Sigma-Aldrich

Sodium chloride, tablet

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

Sigma-Aldrich

Sodium chloride solution, 5 M

Sigma-Aldrich

Nitrilotriacetic acid trisodium salt, Sigma Grade, ≥98%

Sigma-Aldrich

Sodium chloride solution, 1 M

Sigma-Aldrich

Tris(hydroxymethyl)aminomethane, JIS special grade, ≥99.0%

Sigma-Aldrich

Sodium chloride, JIS special grade, ≥99.5%

Sigma-Aldrich

Sodium chloride, SAJ first grade, ≥99.0%