T6066

Trizma^® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• PubChem Substance ID: 24900345
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 400
• Agency: USP/NF; meets EP testing specifications; meets USP testing specifications
• description: aminopeptidase substrate
• Assay: ≥99.9% (titration)
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, protease, none detected; Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested; ≤0.2% water (Karl Fischer)
• loss: ≤0.5% (loss on drying, 110 °C)
• pH: 10.0-11.5
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: soluble (666 mg/ml, yielding a clear colorless solution)
• absorption: ≤0.05 at 260 at 1 M; ≤0.05 at 290 at 40%; ≤0.06 at 280 at 1 M
• application(s): diagnostic assay manufacturing
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma has been used:

• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
• As a component of pre-hybridization buffer in in situ hybridization
• To suspend dried protein before SDS-PAGE protein characterization
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation
• In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Packaging

T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves