93350

Sigma-Aldrich

Trizma® base

puriss. p.a., ≥99.7% (T)

Synonym(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

description

aminopeptidase substrate

grade

puriss. p.a.

assay

≥99.7% (T)

form

crystalline

ign. residue

≤0.01% (as SO4)

pH

10.5-11.5 (25 °C, 10% in H2O)

useful pH range

7 - 9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

water: soluble (678 g/l at 20 °C)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

10 % in H2O

UV absorption

λ: 230 nm Amax: ≤0.6
λ: 260 nm Amax: ≤0.05
λ: 280 nm Amax: ≤0.04
λ: 430 nm Amax: ≤0.01

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based Assay.
Liu Y, et al.
Journal of Bacteriology (2017)
a-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay.
Wordehoff M and Hoyer W
Bio-protocol, 8(14) (2018)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62(1), 61-66 (2002)
Preparation of Buffers for Use in Enzyme Studies.
Gomori G
Methods in Enzymology, 1, 138-146 (1955)
Sambrook J and Russell DW
Molecular Cloning: A Laboratory Manual (2001)

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