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MRCF0R030
Microcon-30kDa Centrifugal Filter Unit with Ultracel-30 membrane
This 0.5 mL centrifugal filter enables the concentration of biomolecules with a membrane NMWL of 30 kDa. Micron centrifugal filters are ideal for DNA & protein concentration & desalting.
Recommended Products
material
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
Quality Level
sterility
non-sterile
feature
holdup volume> 10 μL
manufacturer/tradename
Microcon®
parameter
0.5 mL sample volume
technique(s)
DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable
L
4.50 cm (1.8 in.)
diam.
12.3 mm
filtration area
0.32 cm2
pore size
30 kDa NMWCO
shipped in
ambient
Related Categories
1 of 4
This Item | MRCF0R100ET | MPE300025 | MPE010025 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename - | manufacturer/tradename - |
| pore size 30 kDa NMWCO | pore size - | pore size 300 kDa NMWCO | pore size 10 kDa NMWCO |
| feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL |
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
General description
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X
Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing
Sample applications:
•Recovery of genomic DNA for forensic applications
•Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
•Removal of primers from amplified DNA
•Easy filtrate recovery, exploiting the flat membrane design
Linkage
Legal Information
Storage Class Code
10-13 - German Storage Class 10 to 13
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Articles
In this study, we developed a rapid trypsin digest kit that, at elevated temperatures, yielded reliable, reproducible results in less than 2 hours on a wide variety of substrates for mass spectrometry.
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
Protocols
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
Related Content
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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