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MilliporeSigma

P1754

TWEEN® 80

viscous liquid

TWEEN® 80

Synonym(s):

TWEEN® 80, POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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25 ML

$35.40

500 ML

$96.00

1 L

$125.00

1 GAL

$261.00

$35.40


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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12161900
NACRES:
NA.21

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biological source

Porcine (tallow)
bovine (tallow)

Quality Level

description

non-ionic

form

viscous liquid

mol wt

micellar avg mol wt 79,000
average mol wt 1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

CMC

0.012 mM (20-25°C)

viscosity

400-620 mPa.s

transition temp

cloud point 65 °C

solubility

water: soluble, clear to slightly hazy, colorless to yellow

density

1.064 g/cm3

HLB

15

application(s)

cell analysis

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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This Item
P8074P4780STS0204
form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

aggregation number

60

aggregation number

60

aggregation number

-

aggregation number

-

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

-

biological source

Porcine (tallow), bovine (tallow)

biological source

-

biological source

-

biological source

-

viscosity

400-620 mPa.s

viscosity

-

viscosity

-

viscosity

300—500 cP(25 °C)

density

1.064 g/cm3

density

1.06 g/mL at 25 °C

density

1.06 g/mL at 25 °C (lit.), 1.07 g/cm3 at 25 °C (lit.)

density

1.064 g/cm3

General description

Tween® 80 is a non-ionic detergent and surfactant, classified as an ester of sorbitan and oleic acid. It is a versatile compound commonly employed in various biological applications, including cell lysis, nuclei isolation, and cell fractionation. Additionally, Tween 80 finds use in stabilizing proteins and facilitating protein membrane studies. Its effectiveness stems from its membership in the polysorbate group, characterized by the combination of sorbitol and sorbitane oleic esters with polyethylene glycol chains. These structural features contribute to Tween 80′s emulsifying capabilities and a broad range of applications.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats.[1][2] It has been used for the determination of esterase activity in bacterial cells.[3]

Biochem/physiol Actions

TWEEN® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells.[4][5][6]

Features and Benefits

  • Excellent wetting and dispersing agent
  • Highly versatile surfactant for your Cell Biology and Biochemical research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

>235.4 °F - closed cup

flash_point_c

> 113 °C - closed cup

ppe

Eyeshields, Gloves


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Zandra Gerdes et al.
PloS one, 14(2), e0205378-e0205378 (2019-02-20)
The role of microplastic (MP) as a carrier of persistent organic pollutants (POPs) to aquatic organisms has been a topic of debate. However, the reverse POP transport can occur if relative contaminant concentrations are higher in the organism than in
Pathogenic factors of Pseudomonas cepacia isolates from patients with cystic fibrosis.
Gessner AR and Mortensen JE
Journal of Medical Microbiology, 33, 115-115 (1990)
Davidson JN
The Biochemistry of the Nucleic Acids (2012)
Scopes RK
Protein Purification: Principles and Practice (1993)
Yee-Kuen Yam et al.
Frontiers in microbiology, 11, 359-359 (2020-03-21)
Persistence of infection despite extensive chemotherapy with antibiotics displaying low MICs is a hallmark of lung disease caused by Mycobacterium abscessus (Mab). Thus, the classical MIC assay is a poor predictor of clinical outcome. Discovery of more efficacious antibiotics requires

Questions

11 of 11 Questions  
  1. Is Product P1754, TWEEN® 80, tested for RNase and DNase activity?

    1 answer
    1. No. Product P1754 is not tested for contaminating RNase and DNase activity.  Product P5188 is a Molecular Biology grade product that has been tested for DNase and RNase.

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