This product is not fatty acid free. Currently, a fatty acid free version of this material is not available.
A9771
Albumin–fluorescein isothiocyanate conjugate
protein bovine
Synonym(s):
FITC-albumin
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50 MG
$109.00
100 MG
$197.00
250 MG
$321.00
1 G
$967.00
5 G
$3,930.00
About This Item
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Product Name
Albumin–fluorescein isothiocyanate conjugate, protein bovine
biological source
protein from bovine
form
powder
extent of labeling
≥7 mol FITCmol albumin
technique(s)
ELISA: suitable
solubility
10 mM Tris pH 7.0: 10 mg/mL
application(s)
detection
storage temp.
2-8°C
Quality Level
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Related Categories
1 of 4
This Item | A4737 | A4378 | A1900 |
|---|---|---|---|
| technique(s) ELISA: suitable | technique(s) ligand binding assay: suitable | technique(s) ELISA: suitable | technique(s) SDS-PAGE: suitable |
| solubility 10 mM Tris pH 7.0: 10 mg/mL | solubility H2O: soluble 50 mg/mL | solubility water: soluble (50 mg/ml) | solubility H2O: soluble 50 mg/mL |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) detection | application(s) - | application(s) - | application(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Application
Albumin−fluorescein isothiocyanate conjugate has been used:
- as a control to compare the intracellular delivery efficiency of proteins with or without carrier[1]
- for the fluorescent visualization of proteins and cells[2][3]
- as a blocking agent in ELISA[4]
- as a chemical for brain perfusion and fixation[5]
- for in vivo capillary–epithelial permeability experiment[6]
- for confocal microscopy of right ventricular (RV) vasculature in rats[7]
General description
Albumin makes up 60% of the plasma protein,[8] which is produced by the liver.[9] It regulates colloid osmotic pressure. Albumin is implicated in the transportation, distribution and metabolism of fatty acids, thyroid hormones and drugs.[9] It reduces weight gain, blocks pulmonary edema, decreases ascites and lowers tissue edema.[10]
Preparation Note
Prepared from crystallized and lyophilized albumin (A 4378).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Journal of Molecular Medicine, 95(4), 381-393 (2017)
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The Journal of experimental biology, 212(Pt 14), 2287-2295 (2009-06-30)
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Related Content
Product Information Sheet
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Is this albumin "Fatty Acid Free"?
1 answer-
Helpful?
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Is the conjugate chromophore based 5-fluorescein, 6-fluorescein, or is based on an isomeric (5/6-) mixture?
1 answer-
The chromaphore used to prepare this product is Fluorescein 5-isothiocyanate.
Helpful?
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Is there any free FITC present in Product A9771, Albumin-fluorescein isothiocyanate conjugate?
1 answer-
We would not expect there to be free FITC. The final product is dialyzed against distilled water using dialysis tubing with a molecular weight cutoff of 12,000 prior to it being lyophilized.
Helpful?
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What are the excitation and emission maxima for Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?
1 answer-
The excitation maximum is 495 nm and the emission maximum is 520 nm.
Helpful?
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How is the FITC bound to the albumin in Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?
1 answer-
The FITC is bound through the epsilon amino group of the lysine amino acid residues. Each lot number will have a varying number of FITC molecules bound.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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