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A9771

Sigma-Aldrich

Albumin–fluorescein isothiocyanate conjugate

protein bovine

Synonym(s):
FITC-albumin
MDL number:
eCl@ss:
42010201
NACRES:
NA.32

biological source

protein from bovine

Quality Level

form

powder

extent of labeling

≥7 mol FITCmol albumin

technique(s)

ELISA: suitable

solubility

10 mM Tris pH 7.0: 10 mg/mL

application(s)

detection

storage temp.

2-8°C

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General description

Albumin makes up 60% of the plasma protein, which is produced by the liver. It regulates colloid osmotic pressure. Albumin is implicated in the transportation, distribution and metabolism of fatty acids, thyroid hormones and drugs. It reduces weight gain, blocks pulmonary edema, decreases ascites and lowers tissue edema.

Application

Albumin−fluorescein isothiocyanate conjugate has been used:
  • as a control to compare the intracellular delivery efficiency of proteins with or without carrier
  • for the fluorescent visualization of proteins and cells
  • as a blocking agent in ELISA
  • as a chemical for brain perfusion and fixation
  • for in vivo capillary–epithelial permeability experiment
  • for confocal microscopy of right ventricular (RV) vasculature in rats

Packaging

1, 5 g in poly bottle
50, 100, 250 mg in poly bottle

Preparation Note

Prepared from crystallized and lyophilized albumin (A 4378).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the excitation and emission maxima for Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    The excitation maximum is 495 nm and the emission maximum is 520 nm.

  4. How is the FITC bound to the albumin in Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    The FITC is bound through the epsilon amino group of the lysine amino acid residues. Each lot number will have a varying number of FITC molecules bound.

  5. Is there any free FITC present in Product A9771, Albumin-fluorescein isothiocyanate conjugate?

    We would not expect there to be free FITC. The final product is dialyzed against distilled water using dialysis tubing with a molecular weight cutoff of 12,000 prior to it being lyophilized.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Molecular and Genetic Basis of Renal Disease (2008)
Antonio Brante et al.
The Journal of experimental biology, 212(Pt 14), 2287-2295 (2009-06-30)
Encapsulation is a common strategy among marine invertebrate species. It has been shown that oxygen and food availability independently constrain embryo development during intracapsular development. However, it is unclear how embryos of species with different feeding strategies perceive these two
Ischemia-induced Drp1 and Fis1-mediated mitochondrial fission and right ventricular dysfunction in pulmonary hypertension
Tian L, et al
Journal of Molecular Medicine, 95(4), 381-393 (2017)
George A Tanner et al.
American journal of physiology. Renal physiology, 296(6), F1269-F1278 (2009-04-03)
Many aspects of the glomerular filtration of macromolecules remain controversial, including the location of the major filtration barrier, the effects of electrical charge, and the reason the filtration barrier does not clog. We examined these issues in anesthetized Necturus maculosus
Whole brain vascular imaging in a mouse model of Alzheimer?s disease with two-photon microscopy
Delafontaine-Martel P, et al.
Journal of Biomedical Optics, 23(7), 076501-076501 (2018)

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