C0875

Calcein

Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

• Synonym(s): Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
• Empirical Formula (Hill Notation): C₃₀H₂₆N₂O₁₃
• CAS Number: 154071-48-4
• Molecular Weight: 622.53
• Beilstein: 8181068
• PubChem Substance ID: 24892279
• NACRES: NA.47

Properties

• Quality Level: 200
• description: mixed isomers
• form: powder
• technique(s): titration: suitable
• solubility: NaOH: 50 mg/mL
• fluorescence: λ_ex 470 nm; λ_em 509 nm (pH 7.4)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

Description

Application

Calcein has been used:

• in whole cell staining
• in histomorphometry and micro-computed tomography
• to label active bone-forming surfaces

Packaging

1, 5, 10, 25 g in glass bottle

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves