No in-house testing has been performed to determine if Calcein is membrane permeable or membrane impermeable. The literature suggests Calcein appears to be membrane impermeable. Conn's Biological Stains, 10th Edition, page 250 states, "entry of the dye into cells may be achieved by direct insertion of the hydrophilic calcein, or by the uptake of calcein AM with intracellular formation of calcein."
C0875
Calcein
Powder
Synonym(s):
Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
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About This Item
Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
Molecular Weight:
622.53
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Beilstein/REAXYS Number:
8181068
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Product Name
Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
InChI key
DEGAKNSWVGKMLS-UHFFFAOYSA-N
SMILES string
OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O
description
mixed isomers
form
powder
technique(s)
titration: suitable
color
yellow to brown
solubility
NaOH: 50 mg/mL
fluorescence
λex 470 nm; λem 509 nm (pH 7.4)
suitability
suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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This Item | M1255 | F6377 | M8010 |
|---|---|---|---|
| suitability suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg) | suitability suitable for indicator for EDTA titration | suitability - | suitability - |
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - |
| fluorescence λex 470 nm; λem 509 nm (pH 7.4) | fluorescence λex 322 nm; λem 445 nm in methanol | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence λex 319 nm; λem 384 nm (pH 9.0), λex 360 nm; λem 449 nm (Reaction product), λex 368 nm; λem 505 nm in H2O |
| color yellow to brown | color white to light yellow | color orange to brown | color - |
| solubility NaOH: 50 mg/mL | solubility 1 M NaOH: 50 mg/mL | solubility H2O: 1 mg/mL | solubility acetic acid: 10 mg/mL |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
Application
Biochem/physiol Actions
Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions.[4] Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.[5]
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Calcium: Chemistry, Analysis, Function and Effects (2015)
Skeletal abnormalities in Pth-null mice are influenced by dietary calcium.
Miao D, et al.
Endocrinology, 145, 2046-2046 (2004)
Parathyroid hormone-related peptide is required for increased trabecular bone volume in parathyroid hormone-null mice.
Miao D, et al.
Endocrinology, 145, 3554-3554 (2004)
Kengo Sato et al.
Clinical science (London, England : 1979), 133(16), 1779-1796 (2019-08-11)
Plasma levels of chemerin, an adipocytokine produced from the adipose tissues and liver, are associated with metabolic syndrome and coronary artery disease (CAD). Chemerin and its analog, chemerin-9, are known to bind to their receptor, ChemR23. However, whether chemerin and
Wnt10b deficiency results in age-dependent loss of bone mass and progressive reduction of mesenchymal progenitor cells
Stevens JR, et al.
Journal of Bone and Mineral Research, 25(10), 2138-2147 (2010)
Articles
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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Is this calcein (C0875) membrane-permeable or membrane-impermeable?
1 answer-
Helpful?
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What are the excitation and emission wavelengths of Calcein, Product C0875?
1 answer-
Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the solubility of Calcein, Product C0875?
1 answer-
Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.
Helpful?
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What is the extinction coefficient of Calcein, Product C0875?
1 answer-
For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.
Helpful?
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Is there a reference for using Calcein, Product C0875, to study bone growth?
1 answer-
Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).
Helpful?
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