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C0875

Sigma-Aldrich

Calcein

Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

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Synonym(s):
Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
Molecular Weight:
622.53
Beilstein/REAXYS Number:
8181068
PubChem Substance ID:
NACRES:
NA.47

description

mixed isomers

form

powder

technique(s)

titration: suitable

solubility

NaOH: 50 mg/mL

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

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1 of 4

This Item
M1255F6377F3651
vibrant-m

C0875

Calcein

vibrant-m

M1255

Calcein Blue

vibrant-m

F6377

Fluorescein sodium salt

vibrant-m

F3651

Fluorescein 5(6)-isothiocyanate

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

fluorescence

λex 322 nm; λem 445 nm in methanol

fluorescence

λex 460 nm; λem 515 nm(lit.)

fluorescence

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

form

powder

form

powder

form

powder

form

powder

solubility

NaOH: 50 mg/mL

solubility

1 M NaOH: 50 mg/mL

solubility

H2O: 1 mg/mL

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

Application

Calcein has been used:
  • in whole cell staining
  • in histomorphometry and micro-computed tomography
  • to label active bone-forming surfaces

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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