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C8118

Sigma-Aldrich

Chymase human

recombinant, expressed in Pichia pastoris

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Synonym(s):
Mast cell protease I
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

recombinant

expressed in Pichia pastoris

Quality Level

form

liquid

specific activity

≥40 units/mg protein

mol wt

~37 kDa by SDS-PAGE

concentration

125-400 μg/mL

UniProt accession no.

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CMA1(1215)

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This Item
C4129T0303SAB5700983
Chymase human recombinant, expressed in Pichia pastoris

Sigma-Aldrich

C8118

Chymase human

form

liquid

form

lyophilized powder

form

lyophilized powder

form

liquid

specific activity

≥40 units/mg protein

specific activity

≥40 units/mg protein

specific activity

13,000-20,000 BAEE units/mg protein

specific activity

-

mol wt

~37 kDa by SDS-PAGE

mol wt

25 kDa

mol wt

23.8 kDa

mol wt

-

concentration

125-400 μg/mL

concentration

-

concentration

-

concentration

1.1 mg/mL

UniProt accession no.

P23946

UniProt accession no.

P00767

UniProt accession no.

-

UniProt accession no.

P23946

Application

Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.
Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.

Biochem/physiol Actions

The enzyme rapidly converts angiotensin I to angiotensin II. Optimum pH for the enzyme activity is between 7.5 and 9.0. Enzyme activity is inhibited by soybean trypsin inhibitor, phenylmethylsulfonyl fluoride and chymostatin.

Physical properties

Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.

Unit Definition

One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.

Physical form

Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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I Waern et al.
Mucosal immunology, 6(5), 911-920 (2012-12-14)
Mast cells (MCs) are major effector cells contributing to allergic conditions. When activated, they can release large amounts of active proteases, including chymase from their secretory granules. Here we assessed the role of the chymase mouse mast cell protease 4
Devandir Antonio de Souza et al.
PloS one, 7(7), e40790-e40790 (2012-07-21)
Tumor cells are surrounded by infiltrating inflammatory cells, such as lymphocytes, neutrophils, macrophages, and mast cells. A body of evidence indicates that mast cells are associated with various types of tumors. Although role of mast cells can be directly related
Jacco J de Haan et al.
American journal of physiology. Gastrointestinal and liver physiology, 305(5), G383-G391 (2013-07-03)
Nutritional stimulation of the cholecystokinin-1 receptor (CCK-1R) and nicotinic acetylcholine receptor (nAChR)-mediated vagal reflex was shown to reduce inflammation and preserve intestinal integrity. Mast cells are important early effectors of the innate immune response; therefore modulation of mucosal mast cells
Su Duy Nguyen et al.
Journal of lipid research, 53(10), 2115-2125 (2012-08-03)
HDL particles may enter atherosclerotic lesions having an acidic intimal fluid. Therefore, we investigated whether acidic pH would affect their structural and functional properties. For this purpose, HDL(2) and HDL(3) subfractions were incubated for various periods of time at different
S Takai et al.
Clinica chimica acta; international journal of clinical chemistry, 265(1), 13-20 (1997-11-14)
A chymostatin-sensitive angiotensin II-generating enzyme was found in human gastroepiploic arteries. The enzyme was purified using heparin affinity and gel filtration columns. The molecular mass of the purified enzyme was 30 kDa, and the optimum pH was between 7.5 and

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