C8919

Sigma-Aldrich

Collagen from calf skin

Bornstein and Traub Type I, (0.1% solution in 0.1 M acetic acid), aseptically processed, BioReagent, suitable for cell culture

CAS Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

bovine (calf) skin

sterility

aseptically processed

product line

BioReagent

form

(0.1% solution in 0.1 M acetic acid)

packaging

pkg of 20 mL

availability

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

application(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

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General description

Collagen type I is a component of skin, bone, tendon, and other fibrous connective tissues. It consists of repeating triplet amino acids glycine, proline and hydroxyproline. Collagen is a left handed helix with three polypeptide chains. It is a component of extracellular matrix and as close to 28 types are present in bovine.

Application

Collagen from calf skin has been used:
  • for pre-coting glass slides for immunofluorescence studies
  • as a cell adhesion factor and modification of poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) films for neuron culture
  • for coating culture dishes for murine embryonic fibroblasts culture

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Biochem/physiol Actions

Collagen type I on heat denaturation results in disruption of triple helix to a randomly coil. Mutations in the collagen gene are implicated in a variety of cattle diseases. It has applications in food and cosmetics. Collagen is used as a biomaterial and as a tissue scaffold in tissue engineering. Collagen type I fiber assembly is very crucial for physiological processes and cellular signaling.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Preparation Note

This product is a 0.1% (1mg/ml) solution of calf skin collagen 0.1 M acetic acid. The product′s appearance is turbid, milky white solution. It is recommended that dilutions of product are prepared in sterile water. Please refer to information given on the product information sheet prior to introduction of product to cells and media.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. How can I sterilize collagen solutions?

    We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of collagen solution. DO NOT SHAKE OR STIR. Allow to stand overnight at 2-8 °C. Aseptically remove the top layer containing the collagen solution to a new bottle. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. Can Product C8919, Collagen solution, be used to make 3-D collagen gels?

    This product is NOT intended to produce 3-D (thick) gels, since the solution will not gel. This may be due to gamma-irradiation of the powder during the production process.  We recommend using Product No C4243, which is tested and found to be suitable for 3-D gel formation.

  4. Why does Product C8919, Collagen solution, look milky or contain particulates?

    C8919, Collagen Solution from calf skin, is prepared by allowing irradiated sterile collagen strands to mix with sterile filtered 0.1N acetic acid over several hours. The collagen dissolves to an opalescent solution that is aseptically dispensed while stirring.  No further filtration is necessary.  Due to cross-linkage and sequence variability, as well as structural changes that can occur during isolation and purification, Collagen Type I (Bornstein and Traub) is a heterogeneous mixture rather than a pure compound.  This variability, along with slight differences in the raw material, lends itself to shifts in the opacity of the solution from lot to lot.  This product is tested for sterility prior to release for sale to our customers.  A milky or particulate appearance is not indicative of microbial contamination and does not affect product use. .

  5. How can I use Product C8919, Collagen solution, to coat my cultureware?

    A protocol for coating cultureware can be found on the product information sheet (under Documents, above).

  6. How long can plates coated with Product C8919, Collagen solution, be stored?

    We make no recommendations about storage of plates after coating.  Since factors such as humidity may impact on longevity of coated plates, customers should make their own determination as to whether plates can be stored and under which conditions.  We used to offer SigmaScreen collagen coated plates as Product No. S3315. For optimal performance of that product, we recommend that the unopened product should be stored in a dry place at 2-8°C. We found that the S3315 could be stored at room temperature for up to three months, and that it should never be exposed to temperatures above 50°C.

  7. Product C8919, Collagen solution, gelled while refrigerated.  Can it still be used?

    This can occur but does not harm the product.  When warmed to room temperature, the product will liquify.  Please make sure it is well mixed prior to dilution for coating cultureware.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

Culturing neurons on MEMS fabricated P (VDF-TrFE) films for implantable artificial cochlea
Shintaku H, et al.
Journal of Biomechanical Science and Engineering, 5(3), 229-235 (2010)
Collagen: a network for regenerative medicine
Pawelec KM, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine, 4(40), 6484-6496 (2016)
Hedgehog antagonist cyclopamine isomerizes to less potent forms when acidified
Wilson SR, et al.
Journal of Pharmaceutical and Biomedical Analysis, 52(5), 707-713 (2010)
Extracellular Collagen VI Has Prosurvival and Autophagy Instructive Properties in Mouse Fibroblasts
Castagnaro S, et al.
Frontiers in Physiology, 9(3), 1129-1129 (2018)
Vahid Serpooshan et al.
Scientific reports, 7(1), 12590-12590 (2017-10-05)
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