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CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

for protein expression

All Photos(3)
NACRES:
NA.85

grade

for molecular biology

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

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69450-M69450-MCMC0007
BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

Sigma-Aldrich

69450-M

BL21(DE3) Competent Cells - Novagen

SIG10 5a Chemically Competent Cells for DNA plasmid production

Sigma-Aldrich

CMC0007

SIG10 5a Chemically Competent Cells

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

grade

for molecular biology

grade

-

grade

-

grade

for molecular biology

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

cell transformation

transformation efficiency: >2×107 cfu/μg

cell transformation

transformation efficiency: >2×107 cfu/μg

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 108 cfu/μg

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.

Features and Benefits

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Components

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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