If 0.3% H2O2 (by volume) is to be achieved, it would be necessary to add 0.15 mL of H2O2, which would be 0.5 mL of the 30% solution. If the solution is to be exactly 0.3% H2O2, it would be best to only use 49.5 mL of PBS, to account for the added volume of H2O2 solution. If the 0.3% is to be m/v or v/v, the density would need to be used in order to convert.
H1009
Hydrogen peroxide solution
Hydrogen peroxide solution
30 % (w/w) in H2O, contains stabilizer
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5 ML
$68.10
100 ML
$75.90
500 ML
$196.00
About This Item
Empirical Formula (Hill Notation):
H2O2
CAS Number:
Molecular Weight:
34.01
Beilstein/REAXYS Number:
3587191
MDL number:
UNSPSC Code:
12352304
PubChem Substance ID:
NACRES:
NA.77
Recommended Products
vapor pressure
23.3 mmHg ( 30 °C)
assay
29.0-32.0% (titration)
form
liquid
shelf life
~2 yr
reaction suitability
reagent type: oxidant
concentration
30 % (w/w) in H2O
technique(s)
cell based assay: suitable
immunohistochemistry: suitable
density
1.110 g/cm3
suitability
suitable for DNA precipitation
application(s)
general analytical
pharmaceutical
storage temp.
2-8°C
SMILES string
OO
InChI
1S/H2O2/c1-2/h1-2H
InChI key
MHAJPDPJQMAIIY-UHFFFAOYSA-N
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General description
Hydrogen peroxide (H2O2), a topical antiseptic, is synthesized in aerobic cells as a byproduct of aerobic respiration. It might also be produced as an output of enzymatic reactions in peroxisomes, mitochondria, or other cell compartments.
Application
Hydrogen peroxide solution has been used:
- to treat nasal epithelial cells for H2O2 sensitivity assays
- to treat the human immortal keratinocyte cell line (HaCaT) and the human melanoma cell line (GLL19) to induce senescence
- to incubate brain sections for bromodeoxyuridine (BrdU)/NeuN staining
- in cell proliferation assay to incubate neural stem cells (NE-4C) for in vitro experiments
Biochem/physiol Actions
Hydrogen peroxide (H2O2) is mainly used in wound cleaning. It can kill the pathogens through oxidation burst and by producing local oxygen. H2O2 functions as a signaling molecule or second messenger. It plays a key role in gene expression in numerous ways. H2O2 participates in modulating inflammation.
Features and Benefits
This compound is featured on the Phosphoprotein Phosphatases (Tyrosine) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Disclaimer
The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.
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Danger
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Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1
Storage Class
5.1B - Oxidizing hazardous materials
wgk_germany
WGK 1
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Articles
This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.
Protocols
This procedure may be used for all Catalase products.
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
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How much volume do i need of 30% H2O2 if i want to add 0.3% of it to a 50 ml PBS solution?
1 answer-
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How much we need to add 30% H2O2 in 1mL of solution(water as solvent) to make100mM molarity?
1 answer-
The molarity of this product is calculated to be 9.791 M based on a density of 1.11 g/mL, a formula weight of 34.01 g/mol, and a concentration of 30 w/w%. Here is the calculation:
Molarity = Density x concentration x 1000 / formula weight = 1.11 x 0.3 x 1000 / 34.01 = 9.791 M.To make a 100 mM solution, use the Dilution Formula: C1V1 = C2V2 to find the volume V1 of the 30% H2O2 solution needed.
C1 = 9.791 M (concentration of the stock solution)
C2 = 0.1 M (desired concentration)
V2 = 0.001 L + V1 (final volume, which is the sum of 1 mL of water and the volume of H2O2 added)Rearrange the formula to solve for V1:
9.791 x V1 = 0.1 x (0.001 + V1)
V1 = 0.1 x 0.001 / (9.791 - 0.1) = ~0.0000103 L = ~10.3 µLTherefore, approximately 10.3 µL of this product is required for a 100 mM H2O2 solution when mixed with 1 mL of water.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What stabilizer was added? I can't find any information in the DSD or spec sheet.
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This product contains Dipicolinic acid at 5 – 10ppm as a stabilizer.
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how can we determinate H2O2 concentration in case of 30% by titration , i found way to do this with sodium thiosulfate but they mentioned that is for 0.1% - 5 % con. - can i use it ?
1 answer-
Using a procedure meant for lower concentrations of hydrogen peroxide may lead to less accurate results. Solvay chemicals has a method for determination of the concentration of hydrogen peroxide (20-70%) by titration with potassium permanganate solution: https://www.solvay.com/sites/g/files/srpend221/files/2019-10/H2O2%20Determination%20of%20Hydrogen%20Peroxide%20Concentration%20-20%20to%2070-.pdf
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- CONTACT TIME 문의 해당 과산화수소 수용액 30% 이용하여 소독 진행 시 검증되어 있는 컨택타임이 있는지 문의 드립니다.
1 answer-
Unfortunately, there is no verified contact time for the use of product H1009 in disinfecting.
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Hi, I would like to get information on this product (H2O2 Cat # H1009-5ML) for Lot numbers MKCL6061 and MKCJ1034 to know their expiration dates. Please help me get this information. Thanks.
1 answer-
This product is not assigned an expiration date. It is assigned a recommended retest date of approximately one year after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
Please see the link below to access a specific lot or to review a sample Certificate:
https://www.sigmaaldrich.com/product/sigma/H1009#product-documentationHelpful?
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how much we need to add 30% H2O2 in 7ml of solution(water as solvent) to make 5mM molarity....could you please tell with calculation?
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The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30 % solution of this product can be calculated by the following:
(1110 g/L X 0.30) / 34.01 = 9.79 M.The volume of product required to prepare 7 mL of 0.005 M H2O2 would be 3.6 ul. For ease of pipetting, it may be more practical to prepare a larger volume. For example:
Use 3.6 uL to prepare 7 mL at 5 mM
Use 7.7 uL to prepare 15 mL at 5 mM
Use 10.3 uL to prepare 20 mL at 5 mMAlternatively, an intermediate working solution can be prepared that would require further dilution.
To prepare a 1 M stock from the 9.79 M concentrate, a dilution may be prepared using the M1V1 =M2V2 formula.
In this case, 1.02 mL of the 9.79 M stock is diluted to 10 mL to achieve a 1 M concentration.
From the 1 M solution dilute 0.035 mL (35 ul) to a final volume of 7 mL to achieve a 5 mM concentration.Helpful?
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30% is w/w ratio or its w/v ratio and can you give formula to calculate molarity of this H2O2 30% solution?
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As stated on the product detail page, this is hydrogen peroxide 30% w/w in H2O.
The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30% solution of this product can be calculated by the following:
(1197 g/L X 0.30) / 34.01 = 9.79 MHelpful?
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