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M0650

Sigma-Aldrich

Medium 199

10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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NACRES:
NA.75

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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This Item
M9163M7528M2154
Sigma-Aldrich

Sigma-Aldrich

M0650

Medium 199

Sigma-Aldrich

Sigma-Aldrich

M9163

Medium 199

Medium 199 HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M7528

Medium 199

Sigma-Aldrich

Sigma-Aldrich

M2154

Medium 199

form

liquid

form

liquid

form

liquid

form

liquid

concentration

10 ×

concentration

10 ×

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

components

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

  • to culture human mesothelial cells
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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