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P4417

Sigma-Aldrich

Phosphate buffered saline

tablet

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Synonym(s):
PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

form

tablet

Quality Level

storage condition

dry at room temperature

color

white

pH

7.2-7.6 (1 tablet/200 mL)

solubility

water: soluble

density

1.02 g/cm3 at 20—25 °C
1.03 g/cm3 at 20—25 °C

application(s)

diagnostic assay manufacturing
histology
life science and biopharma

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This Item
P5119P38135P3813
Phosphate buffered saline tablet

P4417

Phosphate buffered saline

Sigma-Aldrich

P5119

Phosphate buffered saline

Sigma-Aldrich

P38135

Phosphate buffered saline

Sigma-Aldrich

P3813

Phosphate buffered saline

form

tablet

form

liquid

form

powder

form

powder

application(s)

diagnostic assay manufacturing
histology
life science and biopharma

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing
life science and biopharma
sample preparation

solubility

water: soluble

solubility

-

solubility

water: soluble

solubility

water: soluble

pH

7.2-7.6 (1 tablet/200 mL)

pH

7.2 (25 °C)

pH

7.4

pH

7.4

General description

Phosphate-buffered saline (PBS) is a widely utilized buffer solution in biological research, valued for its versatility and essential attributes. Notably, its isotonicity closely mirrors the pH, osmolarity, and ion concentrations of the human body, rendering it non-toxic to cells. This pivotal characteristic plays a crucial role in diverse cell biology procedures, including the gentle washing of cells, safe transportation of tissues, and precise dilution of cells and reagents. These applications contribute to the preservation of cell viability and the establishment of a conducive physiological environment. The buffering capacity of PBS, sustained by a phosphate-phosphoric acid equilibrium, establishes a stable pH environment crucial for optimal enzyme activity and various biological processes. This makes PBS an ideal choice for tasks such as preparing reagents, protein dialysis, and immunohistochemistry, ensuring the reproducibility and reliability of experimental results.

Expanding beyond cell biology, PBS demonstrates versatility in applications such as creating custom ELISA buffers for optimal antibody-antigen interactions. Moreover, it serves as a proficient running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS plays a pivotal role in the effective washing of cells and tissues. In summary, PBS stands out as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its profound significance in advancing scientific knowledge and understanding.

application

Phosphate buffered saline has been used-
  • for the production of platinum nanoparticles (NP-Pt) hydrocolloids
  • for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM
  • for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
  • for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
  • for suspension of cell pellets during centrifugation
  • in immunoassay procedures

Features and Benefits

  • High-quality product suitable for multiple research applications
  • Can be used as a buffer component in biological and biochemical applications
  • Highly soluble in water

Reconstitution

One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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M Michaelis et al.
The journal of physical chemistry. B, 123(31), 6694-6704 (2019-07-11)
Although successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over
Simon C Drew et al.
PloS one, 5(12), e15875-e15875 (2011-01-07)
The amyloid-β (Aβ) peptide is the primary component of the extracellular senile plaques characteristic of Alzheimer's disease (AD). The metals hypothesis implicates redox-active copper ions in the pathogenesis of AD and the Cu(2+) coordination of various Aβ peptides has been
Sputum cathelicidin, urokinase plasminogen activation system components, and cytokines discriminate cystic fibrosis, COPD, and asthma inflammation.
Xiao W et al
Chest, 128(4), 2316-2326 (2005)
Wenwen Yu et al.
Food chemistry, 241, 493-501 (2017-09-30)
The conversion of barley starch to sugars is a complex enzymic process. Most previous work concerned the biotechnical aspect of in situ barley enzymes. However, the interactions among the macromolecular substrates and their effects on enzymic catalysis has been little
Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.
Lopez C et al
Food Chemistry, 120.1, 22-33 (2010)

Protocols

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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