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P6186

Sigma-Aldrich

Thidiazuron

suitable for plant cell culture, BioReagent

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Synonym(s):
1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea, TDZ
Empirical Formula (Hill Notation):
C9H8N4OS
CAS Number:
51707-55-2
Molecular Weight:
220.25
Beilstein/REAXYS Number:
1078092
EC Number:
257-356-7
MDL number:
MFCD00078723
UNSPSC Code:
10171502
PubChem Substance ID:
24898652
NACRES:
NA.72

product line

BioReagent

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

SMILES string

O=C(Nc1ccccc1)Nc2cnns2

InChI

1S/C9H8N4OS/c14-9(12-8-6-10-13-15-8)11-7-4-2-1-3-5-7/h1-6H,(H2,11,12,14)

InChI key

HFCYZXMHUIHAQI-UHFFFAOYSA-N

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Application

Thidiazuron has been used:
  • as a component of the proliferation medium to test its effect on the proliferation of embryo-like structures obtained from the post-culture of shoot tips
  • as a growth regulator in Murashige and Skoog (MS) media to culture the nodal segments of Hyssopus officinalis L. seedlings
  • as a cytokinin in induction media to experiment with the hormonal composition

Biochem/physiol Actions

Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin used in micropropagations to supplement media such as Murashige and Skoog medium. TDZ promotes plant organogenesis (shoot regeneration) and plant regeneration. Thidiazuron (TDZ) enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. It is also used as a phenylurea herbicide for controlling weeds in agricultural and nonagricultural practices. Thidiazuron acts as a pesticide by inhibiting photosystem II.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wanwimon Lualon et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 96-100 (2008-04-05)
An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The
María Laura Fontana et al.
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 33(3), 179-186 (2010-01-14)
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic
Mukund Shukla et al.
Methods in molecular biology (Clifton, N.J.), 11013, 279-289 (2012-11-28)
Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of
Anushi A Jahan et al.
Applied biochemistry and biotechnology, 163(7), 851-859 (2010-10-07)
An efficient tissue culture technology has been designed for mass multiplication of Nyctanthes arbor-tristis L. by preculturing nodal explants in thidiazuron (TDZ)-supplemented liquid Murashige and Skoog (MS) media. Direct inoculation of nodal segments on semi-solid MS medium augmented with various
Bi-Hong Feng et al.
Journal of plant physiology, 169(2), 176-182 (2011-11-29)
Embryogenic callus (EC) induced from petioles of alfalfa (Medicago sativa L. cv. Jinnan) on B5h medium turned green, compact and non-embryogenic when the kinetin (KN) in the medium was replaced partially or completely by thidiazuron (TDZ). The application of CoCl₂
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