Tangential Flow Filtration Holders & Systems

Benefiting from our leading bioprocess knowledge and engineering expertise in tangential flow filtration (TFF), we have developed multi-use, single-use, and process development systems incorporating many unique, innovative, and intelligent features. Our systems are designed to meet the success criteria for purification by tangential filtration, including low hold-up volume, maximum volume concentration, and optimal product recovery for enhanced process performance.

Process-scale single-use TFF systems

Mobius^® FlexReady Solution for TFF provides a combination of single-use assemblies (Flexware^® assemblies) and process-ready hardware specifically designed for TFF. It is pre-assembled and pre-tested, integrating easily into any process.

The Mobius^® FlexReady Solution for TFF provides maximum adaptability to your changing process needs, such as varying volume levels. The retentate recycle tank is specifically designed for efficient concentration and diafiltration of proteins. Other key system features include:

• Multi-unit operation, multi-product and multi-scale production for maximized flexibility
• Single-use flowpath, eliminating carryover from previous batches and enabling use with single-use tangential flow filtration devices
• Closed mode of operation is possible with specifically designed flowpath and equipment, allowing to reduce contamination risk while increasing flexibility and efficiency
• Full automation

Process-scale multi-use TFF systems

The fully automated Cogent^® Process-Scale TFF System is designed to separate and purify monoclonal antibodies, vaccines, plasma, and therapeutic proteins. It is ideally suited for both pilot and production scale applications, thereby supporting rapid scale-up from small- to large-scale operations. Other key system features include:

• Modular options for the system configuration that best matches process requirements, while minimizing upfront investment
• Full process automation
• Optimized design and component integration of NovAseptic^® valves and TFF cassette holders for lowest minimum working volume and maximum product recovery
• Processes operated in fed-batch, concentration, total recycle, or single-pass mode.

Process development TFF systems

Our family of small-scale equipment is ideally suited for process development from a few hundred milliliters to several tens of liters. Paired with our Pellicon^® family of tangential flow devices, they ensure linear scalability for your process conditions.

• Cogent^® M1: with its automated TMP/ΔP control, the system can run in fed-batch or diafiltration mode using an optional transfer pump, processing up to 100 L or more based on product and membrane filter characteristics, with very low hold-up volume and maximum product recovery.
• Cogent^® µScale: this semi-automated system has been designed with “micro-scale” process development in mind, featuring an extremely low minimum working volume, high feed pressures, and low pulsation pump.
• Labscale: easy to operate, this system is ideally suited for use with Pellicon^® XL devices, at volumes from 50 mL to 1,000 mL. It features the necessary components to run a successful tangential flow filtration screening and process development, with very little volume available.